Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 573462

What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity


Šrut, Maja; Štambuk, Anamaria; Klobučar, Goran Igor Vinko
What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity // Toxicology in vitro, 27 (2013), 4; 1226-1232 doi:10.1016/j.tiv.2013.02.007 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 573462 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity

Autori
Šrut, Maja ; Štambuk, Anamaria ; Klobučar, Goran Igor Vinko

Izvornik
Toxicology in vitro (0887-2333) 27 (2013), 4; 1226-1232

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
RTG-2 cell line; Comet assay; AFLP; B[a]P; EMS

Sažetak
DNA damage detected by genotoxicity biomarkers such as the Comet assay is not always a reliable indicator of the consequences that genotoxic agents can have on the genome integrity of the exposed organisms. Therefore, to reveal the existence of more permanent alterations of DNA structure after genotoxic stress, the RTG-2 rainbow trout cell line was exposed for 3 days to benzo[a]pyrene (B[a]P, 0.1-10 µM) and ethyl methanesulfonate (EMS, 0.1-1 mM) followed by 3 days of recovery period. Primary DNA damage was evaluated by the Comet assay and DNA alterations were assessed using AFLP (amplified fragment length polymorphism). Qualitative and quantitative modifications in AFLP profiles were analyzed in order to detect genetic alterations arising from mutation events and/or DNA damage. Significant induction in DNA damage measured by the Comet assay was noticed after B[a]P treatment at all concentrations but values returned to the control level after recovery. Exposure to EMS induced significant DNA damage only at the highest concentration and damage persisted after the recovery period. AFLP profiles detected DNA alterations even when Comet assay indicated complete DNA repair, revealing more persistent damage. Since such DNA damage can impair its structure and function, Comet assay results should preferably be supplemented with other methods in order to predict the consequences of genotoxic insult more accurately.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Maja Šrut (autor)

Avatar Url Anamaria Štambuk (autor)

Avatar Url Goran Klobučar (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com ac.els-cdn.com dx.doi.org

Citiraj ovu publikaciju:

Šrut, Maja; Štambuk, Anamaria; Klobučar, Goran Igor Vinko
What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity // Toxicology in vitro, 27 (2013), 4; 1226-1232 doi:10.1016/j.tiv.2013.02.007 (međunarodna recenzija, članak, znanstveni)
Šrut, M., Štambuk, A. & Klobučar, G. (2013) What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity. Toxicology in vitro, 27 (4), 1226-1232 doi:10.1016/j.tiv.2013.02.007.
@article{article, author = {\v{S}rut, Maja and \v{S}tambuk, Anamaria and Klobu\v{c}ar, Goran Igor Vinko}, year = {2013}, pages = {1226-1232}, DOI = {10.1016/j.tiv.2013.02.007}, keywords = {RTG-2 cell line, Comet assay, AFLP, B[a]P, EMS}, journal = {Toxicology in vitro}, doi = {10.1016/j.tiv.2013.02.007}, volume = {27}, number = {4}, issn = {0887-2333}, title = {What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity}, keyword = {RTG-2 cell line, Comet assay, AFLP, B[a]P, EMS} }
@article{article, author = {\v{S}rut, Maja and \v{S}tambuk, Anamaria and Klobu\v{c}ar, Goran Igor Vinko}, year = {2013}, pages = {1226-1232}, DOI = {10.1016/j.tiv.2013.02.007}, keywords = {RTG-2 cell line, Comet assay, AFLP, B[a]P, EMS}, journal = {Toxicology in vitro}, doi = {10.1016/j.tiv.2013.02.007}, volume = {27}, number = {4}, issn = {0887-2333}, title = {What is Comet assay not telling us: AFLP reveals wider aspects of genotoxicity}, keyword = {RTG-2 cell line, Comet assay, AFLP, B[a]P, EMS} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font