engleski

Occurrence and antibiotic susceptibility profiles of Burkholderia cepacia complex in coastal marine environment

During an environmental study of bacterial resistance to antibiotics in coastal waters of the Kaštela Bay, Adriatic Sea, Croatia, 47 Burkholderia cepacia complex (Bcc) isolates were recovered from seawater and mussel (Mytillus galloprovincialis) samples. All isolates were multidrug resistant. Two B. cenocepacia isolates produced chromosomally encoded TEM-116 extended- spectrum β-lactamase (ESBL). Analysis of outer membrane proteins revealed that decreased expression of a 36 kDa protein could be associated with high level of β-lactam resistance in both isolates. Phenotypic study of efflux systems also indicated an over-expression of RND efflux- mediated mechanism in one of the isolates. This study demonstrated the presence of Bcc in seawater and M. galloprovincialis from the Kaštela Bay which gives evidence that coastal marine environment, including mussels, could be considered as a reservoir for Bcc species. Detection of ESBL-encoding genes indicates the potential role of these bacteria in the maintenance and dispersion of antibiotic resistance genes.

Burkholderia cepacia complex ; TEM-116 ; ESBL ; Mytilus galloprovincialis ; coastal marine environment

nije evidentirano