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A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids


Wu, Junchen; Zou, Ying; Sicking, Wilhelm; Piantanida, Ivo; Yi, Tao; Schmuck, Carsten
A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids // Journal of the American Chemical Society, 134 (2012), 4; 1958-1961 doi:10.1021/ja2103845 (međunarodna recenzija, članak, znanstveni)


Naslov
A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids

Autori
Wu, Junchen ; Zou, Ying ; Sicking, Wilhelm ; Piantanida, Ivo ; Yi, Tao ; Schmuck, Carsten

Izvornik
Journal of the American Chemical Society (0002-7863) 134 (2012), 4; 1958-1961

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Peptide beacon; DNA sensing; bioorganic chemistry; cell imaging

Sažetak
In conclusion, we have demonstrated that the pyrenefunctionalized oligopeptide 1 can be used as a molecular peptide beacon to sense ds-DNA. Upon binding to nucleic acids 1 undergoes a conformational change from a folded to an extended form which switches the fluorescence of 1 from an excimer emission to a monomer emission. PB 1 can thus be used for the ratiometric sensing of different types of polynucleotides as binding to p(dA•dT)2 is preferred to G-C containing polynucleotides. Most importantly PB 1 is taken up by cells and can thus be used for the staining of nuclear DNA using fluorescence microscopy. As 1 does not possess any general cytotoxicity it might be of interest for applications in diagnostics.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982914-2918 - Dizajn, sinteza i ispitivanje interakcija malih molekula s DNA, RNA i proteinima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Ivo Piantanida, (202026)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI


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