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HISTOPATHOLOGICAL AND IMMUNOHYSTOCHEMICAL EVALUATION OF LEVATOR APONEUROSIS IN APONEUROTIC PTOSIS (CROSBI ID 477574)

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Kuzmanović, Biljana HISTOPATHOLOGICAL AND IMMUNOHYSTOCHEMICAL EVALUATION OF LEVATOR APONEUROSIS IN APONEUROTIC PTOSIS // Abstracts Book of 18th Meeting of European Society of Ophthalmic Plastic and Reconstructive Surgery (E.S.O.P.R.S), Paris, 14-16 September, 2000. / J.A. Bernard, C. Crouzilhac-Bernard, B. Fayet, B. Godgrand (ur.). Pariz: E.S.O.P.R.S, 2000. str. 9-x

Podaci o odgovornosti

Kuzmanović, Biljana

engleski

HISTOPATHOLOGICAL AND IMMUNOHYSTOCHEMICAL EVALUATION OF LEVATOR APONEUROSIS IN APONEUROTIC PTOSIS

Purpose: Histopathological and immunohystochemical evaluation of levator aponeurosis in patients with aponeurotic ptosis vrs aponeurosis of cadavers without history of ptosis, especially in relation with presence of amyloid deposition in the aponeurosis. Material and methods: 7 levator specimens were taken from 6 patients, age 37-78 years, undergoing anterior levator repair due to aponeurotic ptosis. The control group consisted of 12 fresh levator aponeurosis specimens harvested from both lids of 6 cadavers, age 67-80 years, during autopsy. Staining of the paraffin-embedded 6-8ľm sections included hematoxylin-eosin, Masson trichrome followed by Congo red that imparts an orange pink tint to the amyloid deposit and Orcein stain for elastic fibers. Immunohistochemistry was performed as a final marker for amyloid. Results: The levator aponeurosis was attenuated, stretched and grayish in all patients, almost transparent in one, but still attached to the tarsus. Collagen bundles are thinned with space between the bundles filled with abundant fat. Elastic fibers are demonstrated in all samples. Amyloid is positive in 37-year-old female with ptosis due to chronic edema and 78-year-old female with involutive aponeurotic ptosis. Further clinical and laboratory investigations for amyloidosis gave no evidence to suggest orbital or systemic involvement. Amyloid is negative and elastic fibers positive in all samples of the control group. Histopathological characteristics of senile changes in levator aponeurosis of the control group are described. Conclusions: Positive amyloid in levator aponeurosis of 33,3% randomly chosen, relatively small number of patients with aponeurotic ptosis, without systemic amyloidosis, opens a new perspective in aponeurotic ptosis etiology explanation.

immunohystochemistry; levator aponeurosis; aponeurotic ptosis

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Podaci o prilogu

9-x.

2000.

objavljeno

Podaci o matičnoj publikaciji

Abstracts Book of 18th Meeting of European Society of Ophthalmic Plastic and Reconstructive Surgery (E.S.O.P.R.S), Paris, 14-16 September, 2000.

J.A. Bernard, C. Crouzilhac-Bernard, B. Fayet, B. Godgrand

Pariz: E.S.O.P.R.S

Podaci o skupu

18th Meeting of European Society of Ophthalmic Plastic and Reconstructive Surgery (E.S.O.P.R.S), Paris, 14-16 September, 2000.

predavanje

14.09.2000-16.09.2000

Pariz, Francuska

Povezanost rada

Kliničke medicinske znanosti