Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 56651

Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration


Schroeder, Iris; Vasić-Rački, Đurđa; Wandrey, Christian; Liese, Andreas
Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration // Reactive intermediates in organic and biological electrochemistry in honor of late professor Eberhard Steckhan / Yoshida, J. ; Peters, D.G. ; Workentin, M.S. (ur.).
Washington: The Electrochemical Society, 2001. str. 00-00 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 56651 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration

Autori
Schroeder, Iris ; Vasić-Rački, Đurđa ; Wandrey, Christian ; Liese, Andreas

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Reactive intermediates in organic and biological electrochemistry in honor of late professor Eberhard Steckhan / Yoshida, J. ; Peters, D.G. ; Workentin, M.S. - Washington : The Electrochemical Society, 2001, 00-00

Skup
The 199th Meeting of The Electrochemical Society

Mjesto i datum
Sjedinjene Američke Države, 25.03.2001. - 29.03.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
enantioselective enzymatic oxidation; electrochemical NAD regeneration

Sažetak
For production of 2-hydroxyketones and racemic resolution of vicinal diols glycerol dehydrogenase (GDH) [EC 1.1.1.6] from Cellulomonas species was used as production enzyme.1 Since GDH is NAD + dependent and strongly inhibited by its reduced form NADH, an effective and cost efficient cofactor regeneration is necessary. For this regeneration ABTS+.was used as oxidizing agent, which itself was prepared in situ by anodic oxidation. Though electrochemical cofactor regeneration needs no cosubstrate nor additional enzymes, until now it is not used in industrial biotransformations.2, 3 The kinetic parameters were determined and a kinetic model for the electrochemically driven enzymatic reaction was developed. Based on this model the ratios of the reactants were optimized and the model proved by experiments. Synthesis of 2-hydroxyketones and racemic resolution were performed in different reactor types, avoiding product inhibition by continuous extraction of the formed 2-hydroxyketones. GDH was retained by using ultrafiltration membranes. Lit: 1 A. Liese, M. Karutz, J. Kamphuis, C. Wandrey, U. Kragl, Biotech. Bioeng. 1996, 51, 544-550 2 A. Liese, K. Seelbach, C. Wandrey, Industrial Biotransformations, VCH Weinheim 2000 3 E. Steckhan, Top. Curr. Chem 1994, 170, 83-111 Financial support by BASF AG Ludwigshafen (Germany) is gratefully acknowledged.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija



POVEZANOST RADA


Projekti:
125021

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Đurđa Vasić-Rački (autor)


Citiraj ovu publikaciju:

Schroeder, Iris; Vasić-Rački, Đurđa; Wandrey, Christian; Liese, Andreas
Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration // Reactive intermediates in organic and biological electrochemistry in honor of late professor Eberhard Steckhan / Yoshida, J. ; Peters, D.G. ; Workentin, M.S. (ur.).
Washington: The Electrochemical Society, 2001. str. 00-00 (poster, međunarodna recenzija, sažetak, znanstveni)
Schroeder, I., Vasić-Rački, Đ., Wandrey, C. & Liese, A. (2001) Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration. U: Yoshida, J., Peters, D. & Workentin, M. (ur.)Reactive intermediates in organic and biological electrochemistry in honor of late professor Eberhard Steckhan.
@article{article, author = {Schroeder, Iris and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Wandrey, Christian and Liese, Andreas}, year = {2001}, pages = {00-00}, keywords = {enantioselective enzymatic oxidation, electrochemical NAD regeneration}, title = {Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration}, keyword = {enantioselective enzymatic oxidation, electrochemical NAD regeneration}, publisher = {The Electrochemical Society}, publisherplace = {Sjedinjene Ameri\v{c}ke Dr\v{z}ave} }
@article{article, author = {Schroeder, Iris and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Wandrey, Christian and Liese, Andreas}, year = {2001}, pages = {00-00}, keywords = {enantioselective enzymatic oxidation, electrochemical NAD regeneration}, title = {Enantioselective enzymatic oxidation with indirect electrochemical NAD+ regeneration}, keyword = {enantioselective enzymatic oxidation, electrochemical NAD regeneration}, publisher = {The Electrochemical Society}, publisherplace = {Sjedinjene Ameri\v{c}ke Dr\v{z}ave} }




Contrast
Increase Font
Decrease Font
Dyslexic Font