engleski

Chemical composition and evaluation of acetylcholinesterase inhibition and antioxidant activity of essential oil from Dalmatian endemic species <i>Pinus nigra</i> Arnold ssp. <i>dalmatica</i> (Vis.) Franco

In the present study, the chemical composition and biological activity (acetylcholinesterase inhibition and antioxidant activity) of essential oil from endemic species <i>Pinus nigra</i> Arnold ssp. <i>dalmatica</i> (Vis.) Franco was investigated. α-Pinene, β-pinene, germacrene D and β-caryophyllene were identified by GC-FID and GC-MS as dominant components of the oil. <i>P. nigra</i> ssp. <i>dalmatica</i> essential oil showed relatively high inhibitory activity on acetylcholinesterase (AChE), which can be in relation with good AChE inhibitory activity of pure main monoterpene components of the oil (S-α-pinene) and their mixtures (S-α-pinene:S-β-pinene and R-α-pinene:S-β-pinene, in ratio 3:2). Essential oil from <i>P. nigra</i> ssp. <i>dalmatica</i> did not show significant antioxidant activity tested by three methods: DPPH radical scavenging method, Ferric Reducing Antioxidant Potential (FRAP) and Thiobarbituric Acid Reactive Species (TBARS) assays.

Acetylcholinesterase inhibition ; antioxidant activity ; essential oil ; <i>Pinus nigra</i> ssp. <i>dalmatica</i> ; endemic species

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