engleski

Validation of a microscale extraction and highthroughputUHPLC-QTOF-MS analysis method for huperzine A in Huperzia

Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase inhibiting alkaloid, from as little as 3mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20–10, 000pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources.

alkaloid; Huperzia; huperzine A; mass spectrometry; microscale extraction

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