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Pregled bibliografske jedinice broj: 560570

Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response


Slavuljica, Irena; Busche, Andreas; Babić, Marina; Mitrović, Maja; Gašparović, Iva; Cekinović, Đurđica; Markova Car, Elitza; Pernjak Pugel, Ester; Ciković, Ana; Juranić Lisnić, Vanda et al.
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response // 12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts / / Jonjić, Stipan ; Krmpotić, Astrid ; Watzl, Carsten (ur.). (ur.).
Rijeka, 2010. str. 78-78 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response

Autori
Slavuljica, Irena ; Busche, Andreas ; Babić, Marina ; Mitrović, Maja ; Gašparović, Iva ; Cekinović, Đurđica ; Markova Car, Elitza ; Pernjak Pugel, Ester ; Ciković, Ana ; Juranić Lisnić, Vanda ; Messerle, Martin ; Krmpotić, Astrid ; Jonjić, Stipan.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts / / Jonjić, Stipan ; Krmpotić, Astrid ; Watzl, Carsten (ur.). - Rijeka, 2010, 78-78

Skup
12th meeting of the Society for natural immunity and NK 2010,

Mjesto i datum
Cavtat-Dubrovnik, Croatia, 11.-15. September 2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MCMV; NKG2D; RAE-1gamma;

Sažetak
Cytomegalovirus (CMV) is a major viral cause of morbidity and mortality after congenitaly infected and immunocompromised individuals, such as transplant recipiens and HIV-infected pacients. This is why developing an effective and safe HCMV vaccine is utmost importance. NKG2D is a potent activating receptor expressed by the cells of innate and adaptive immunity. Its importance in CMV immunesurveillance is indicated by the elaborative viral evasion mechanisms evolved to avoid NKG2D. In order to study this powerful signaling pathway we designed a recombinant mouse CMV expressing the high affinity NKG2D ligand RAE-1γ (RAE-1γMCMV). Expression of RAE-1γ by MCMV resulted in profound virus attenuation in vivo and lower latent virus DNA loads. RAE-1γMCMV infection was efficiently controlled by immunodeficient hosts, including mice lacking type I interferons receptor and mice immunosuppressed by sublethal γ-irradiation. Features of neonatal RAE-1γMCMV infection were also diminished. Despite tight innate immune control, RAE-1γMCMV infection elicited strong, long-lasting protective immunity. Moreover, upon maternal vaccination with MCMV expressing NKG2D ligand, transplacental transfer of antiviral antibodies protected neonatal mice from CMV disease. RAE-1γ transgene exhibited no sequence variation throughout infection despite of selective pressure. Live, attenuated viruses have large potential to serve as a vaccine vectors. The capacity of recombinant RAE-1γMCMV to serve as a vaccine vector for other viral and bacterial pathogens is currently under investigation. Altogether, our results indicate that a recombinant virus encoding a ligand for NKG2D receptor might provide a new, powerful approach for development of a safe and immunogenic CMV vaccine as well as CMV-based vaccine vector.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
062-0621261-1263 - Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru (Stipan Jonjić, )
062-0621261-1268 - Uloga imunosubverzivnih citomegalovirusnih gena u latenciji (Astrid Krmpotić, )
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Ester Pernjak-Pugel, )

Ustanove
Medicinski fakultet, Rijeka

Citiraj ovu publikaciju

Slavuljica, Irena; Busche, Andreas; Babić, Marina; Mitrović, Maja; Gašparović, Iva; Cekinović, Đurđica; Markova Car, Elitza; Pernjak Pugel, Ester; Ciković, Ana; Juranić Lisnić, Vanda et al.
Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response // 12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts / / Jonjić, Stipan ; Krmpotić, Astrid ; Watzl, Carsten (ur.). (ur.).
Rijeka, 2010. str. 78-78 (poster, međunarodna recenzija, sažetak, znanstveni)
Slavuljica, I., Busche, A., Babić, M., Mitrović, M., Gašparović, I., Cekinović, Đ., Markova Car, E., Pernjak Pugel, E., Ciković, A. & Juranić Lisnić, V. (2010) Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response. U: Jonjić, S., Krmpotić, A. & Watzl, C. (ur.)12th meeting of the Society for natural immunity and NK 2010, 11.-15. September 2010 Cavtat-Dubrovnik, Book of abstracts /.
@article{article, year = {2010}, pages = {78-78}, keywords = {MCMV, NKG2D, RAE-1gamma, }, title = {Immunobiology of recombinant CMV Strain expressing ligand for NKG2D receptor - a dramatic attenuation in vivo does not prevent an efficient and long-lasting protective immune response}, keyword = {MCMV, NKG2D, RAE-1gamma, }, publisherplace = {Cavtat-Dubrovnik, Croatia} }