engleski

Cryosections: Standard staining and immunohistochemistry

Design of practical: Handling tissue for cryosections and immunohistochemistry 1. Introduction: Manual for tissue preparation, fixation methods and cryoprotection of various specimens (I ; 30 min) 2. Preparation of slides for cryosectioning (I ; 10 min) 3. Cryosectioning of various specimens, mounted and free-floating sections ; practical work with each student (I ; 30 min) 4. Basic immunohistochemistry of different molecules (myelin molecules, indirect staining for ganglioside GD1a, fluorescent and enzyme labeled secondary antibodies) (N ; 80 min) 5. Contrasting of nuclei (DAPI) and mounting (N ; 10 min) 6. Microscopy of slides on fluorescent and light microscope (M ; 20 min)

cryosections; immunohystochemistry; artefacts

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