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Quasispecies changes detected in hypervariable region 1 of hepatitis C genome with high-resolution DNA melting analysis in treated and untreated chronic hepatitis C patients


Hrstić, Irena; Grahovac, Blaženka: Mohar, Bojana; Kolarić, Branko; Ostojić, Rajko; Vucelić, Boris.
Quasispecies changes detected in hypervariable region 1 of hepatitis C genome with high-resolution DNA melting analysis in treated and untreated chronic hepatitis C patients // Aabstracts of the ... ; u: Journal of hepatology 52 (2010) (S1) P756, 2010. str. S294-S295 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Quasispecies changes detected in hypervariable region 1 of hepatitis C genome with high-resolution DNA melting analysis in treated and untreated chronic hepatitis C patients

Autori
Hrstić, Irena ; Grahovac, Blaženka: Mohar, Bojana ; Kolarić, Branko ; Ostojić, Rajko ; Vucelić, Boris.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Aabstracts of the ... ; u: Journal of hepatology 52 (2010) (S1) P756 / - , 2010, S294-S295

Mjesto i datum
,

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Quasispecies changes; hypervariable region 1; hepatitis C

Sažetak
HCV exists in an individual as pool of closely related but genetically distinct variants. Development of resistant variants can be the reason of treatment failure. Aim of this study was to determine the changes in HCV quasispecies in region susceptible for mutations. Quasispecies were detected by new method, highresolution DNA melting analysis, in hypervariable region-1 at two points: 1. forty untreated patients: at baseline and after 6 months ; 2. sixty-nine treated patients: at baseline and at the end of treatment. Second sample was collected for all untreated and for 24 treated patients (45 had ETVR). For each sample result was shown as melting curve with 4 parameters: Tm (melting temperature), H (high), W (wide) and A (area). The most common genotype was 1 in both groups (57.5% in untreated and 83.3% in treated). Obtained baseline Tm was statistically significantly correlated with genotype obtained with commercial kits (p = 0.05). No significance was found for other baseline parameters. No statistical significances have been found in Tm, H, W and A for untreated and treated patients between paired samples but medians have changed in both groups (table 1 and 2).Trend of changes differed between groups, in untreated patients W and H raised while in treated declined. Without treatment, quasispecies become more heterogeneous while therapy makes them more homogeneous, towards resistant variants. Since no significant changes have been found in Tm it might be concluded that all observed changesdeveloped between same genotype at baseline.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0000000-3550 - Molekularni mehanizmi infekcije virusom hepatitisa C (Blaženka Grahovac, )

Ustanove
Hrvatski zavod za javno zdravstvo,
Medicinski fakultet, Rijeka,
Klinički bolnički centar Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE