engleski

Identification and validation of a major QTL affecting leaf cadmium concentration in maize

Cadmium (Cd) is heavy metal toxic to humans, animals and plants at very low concentrations. Mobility and availability of Cd to crop plants pose risk to the food chain. Improved maize genotypes with effective ability to accumulate Cd can play an important role in treating Cd- contaminated soil. Our previous study conducted on a maize mapping population B84×Os6-2 identified a major quantitative trait locus (QTL) for leaf Cd concentration on chromosome 2 (bin 2.06). The LOD score for the QTL of 32.5 indicated very high probability that this QTL strongly affected amounts of Cd in leaves. In order to validate this QTL, we analyzed 207 recombinant inbred lines of intermated B73×Mo17 (IBM) population with 636 molecular markers. Our results validate the presence of a major QTL for leaf Cd accumulation with the LOD score of 20.0 at the same position on bin 2.06. We detected a putative candidate gene of known function on the basis of co-location with the major QTL. Our findings could aid rapid development of maize genotypes with decreased/increased Cd accumulation in leaves by manipulation of the gene. Using the detected gene in maize breeding via the new cisgenic approach will be discussed.

cadmium ; cisgenesis ; leaf ; maize ; QTL

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