engleski

Multinucleated giant cell appearance after whole body microwave irradiation on rats

Multinucleated giant cells are common for some chronic inflammatory processes in the lung. These cells are formed by fusion of macrophages, but how the process relates to the kinetics of alveolar macrophage generation is not clear. This study investigated the influence of 2450 MHz microwave irradiation on alveolar macrophage kinetics and formation of multinucleated giant cell after whole body irradiation of rats. The range of electromagnetic radiation was selected as 2450 MHz microwaves at power density of 5 - 15 mW/cm2. A group of experimental animals was divided in four subgroups which received 2x2, 8x2, 13x2 and 22x2 hours of irradiation treatment. The animals were killed on days 1, 8, 16, and 30. Free lung cell population was obtained by bronchoalveolar lavage. Cell response to the selected irradiation level was followed quantitatively, qualitatively and morphologically using standard laboratory methods. Total cell number retrieved by lavage slightly decreased in treated animals showing time- and dose-dependence. Cell viability did not significantly changed in the irradiated animal group (G2) as compared with control group (G1). Multinucleated cells significantly increased in treated animals. The elevation of the number of nuclei per cell was time- and dose- dependent. Macrophages with two nucleoli were more common in the earlier phases of irradiation that is in the first and eight two-hour treatments (2x2 and 8x2 hours of exposure). Polynucleation, that is three and more nucleoli in a single cell, was frequently observed on slides obtained after 13x2 and 22x2 hours of irradiation. Binucleation and multinucleation of alveolar macrophages were sensitive time- and dose- dependent morphological indicators of pulmonary stress.

Wistar rats; irradiation; 2450 MHz microwave; alveolar macrophage

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