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Characterization and chromosome location of repetitive DNA in Anemone hortensis L.

Karyotype of the Mediterranean species Anemone hortensis L. consists of three acrocentrics (chromosomes 1, 2 and 3), one meta-submetacentric (chromosome 4) and four metacentrics (chromosomes 5, 6, 7 and 8). In A. hortensis, large AT-rich heterochromatic bands are located intercalary and terminally on long arm of all acrocentrics and terminally on chromosomes 4 and 5. In this study, we report the nucleotide sequences and chromosomal organization of repetitive elements. The tandem repeats isolated after digestion of genomic DNA with EcoRV (JME) and HindIII (JMH2) were 567 bp and 743 bp long, respectively. The JME satellite sequence family was located in all intercalary DAPI bands, while the JMH2 satellite sequence family coincided to terminal DAPI bands. After digestion of genomic DNA with HindIII, 945 bp long JMH1 repeat sequence was isolated. FISH revealed dispersed organization of JMH1 on all chromosomes of A. hortensis. JMH1 exhibited partial homology to the Ty3/gypsy-like retroelement and has to be confirmed as a part of a retrotransposon.

Anemone hortensis; satellite DNA; dispersed DNA; fluorescent in situ hybridization (FISH)

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