Antioxidant enzyme status in nod mouse embryos during diabetic pregnancy MOUSE EMBRYOS DURING DIABETIC PREGNANCY (CROSBI ID 581120)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Korolija, Marina ; Popović Hadžija, Marijana ; Sobočanec, Sandra ; Mačak Šafranko, Željka ; Hadžija, Mirko
engleski
Antioxidant enzyme status in nod mouse embryos during diabetic pregnancy MOUSE EMBRYOS DURING DIABETIC PREGNANCY
Maternal diabetes increases the rate of congenital malformations in embryos, causing diabetic embryopathy which mostly affects heart and the neural tube. Although the cellular and molecular mechanisms of diabetic embryopathy are poorly understood, it was shown that maternal hyperglycemia induces oxidative stress in embryos. We employed non-obese diabetic mouse strain as an animal model in order to evaluate the incidence of neural tube defects (NTDs) and establish whether or not the antioxidant enzyme status in embryos changes during diabetic pregnancy. We compared E (embryonic day) 10.5 embryos from non-diabetic and alloxan-induced diabetic pregnancies. We assayed mRNA and protein expression, as well as the activity of major antioxidant enzymes that scavenge superoxide (CuZn-superoxide dismutase - Sod1, Mn-superoxide dismutase - Sod2) and catabolize subsequently produced hydrogen peroxide (catalase – Cat, glutathione peroxidase 1 - Gpx1). Methods: Gene expression was analyzed by real-time PCR. Protein quantification was performed using SDS-PAGE and Western blotting. Enzyme activities were determined spectrophotometrically. According to external morphology, we found exencephaly as the most common NTD, affecting nearly 10% of all embryos developed under high glucose conditions. Compared to embryos of healthy mothers, no alteration in mRNA content of sod1, sod2, cat and gpx1 was detected. However, significant increase in Sod2 and Gpx1 protein level and activity was observed. Antioxidant enzyme gene expression does not reflect oxidative stress, while quantity and activity changes in antioxidant enzymes indicate excessive reactive oxygen species production and increased demand for its detoxification in embryos exposed to maternal diabetes.
diabetic embryopathy; oxidative stress; neural tube defects; non-obese diabetic mice
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Podaci o prilogu
1-1.
2011.
objavljeno
Podaci o matičnoj publikaciji
The 6th international symposium on diabetes and pregnancy : book of abstracts
Salzburg:
Podaci o skupu
International symposium on diabetes and pregnancy (6 ; 2011)
poster
23.03.2011-26.03.2011
Salzburg, Austrija