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Pregled bibliografske jedinice broj: 550768

Occurrence of aflatoxin B1, sterigmatocystin and ochratoxin A in olive oil and their production on black olives


Šegvić Klarić, Maja; Zubčić, Snježana; Kosalec, Ivan; Marić, Marko; Herceg, Matej; Matek Sarić, Marijana; Tomić, Siniša
Occurrence of aflatoxin B1, sterigmatocystin and ochratoxin A in olive oil and their production on black olives // Power of Microbes in Industry and Environment
Zagreb, 2010. (poster, nije recenziran, sažetak, znanstveni)


Naslov
Occurrence of aflatoxin B1, sterigmatocystin and ochratoxin A in olive oil and their production on black olives

Autori
Šegvić Klarić, Maja ; Zubčić, Snježana ; Kosalec, Ivan ; Marić, Marko ; Herceg, Matej ; Matek Sarić, Marijana ; Tomić, Siniša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes in Industry and Environment / - Zagreb, 2010

Skup
Power of Microbes in Industry and Environment

Mjesto i datum
Malinska, Hrvatska, 22-25.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Aspergillus; olive; aflatoxin; ochratoxin; sterigmatocystin

Sažetak
Olives can be contaminated with various moulds particularly Aspergillus species. Several reports showed that olives support growth of mycotoxin-producing aspergilli as well as production of aflatoxin (AFB1) and ochratoxin A (OTA) during storage period. Therefore, these and other lipid soluble mycotoxins could be transferred into olive oil. The aim of this study was to determine the presence of AFB1, OTA and sterigmatocystin (STC) in olive oil samples (N=48) collected in 2009 from several producers in Dalmatia (Croatia). Production ability of Aspergillus mycotoxin-producing strains on pickled black olives as substrate was also checked. Upon methanol (80%) extraction, HPLC (UV Vis or FLD detection) was employed for analysis of oil samples as well as black olives that were used as substrate for mycotoxin biosynthesis. 52% of oil samples were contaminated with STC (0.003-0.028 g/mL) while two samples (4%) contained STC in concentrations below limit of quantification (LOQ). AFB1 recovered from 12.5% of samples (0.772-3.104 g/mL), while OTA was detected only in one oil sample and it was below LOQ. STC and AFB1 co-occurred in 6.25% of samples. Mycotoxin-producing Aspergillus strains were able to produce AFB1 (17.35 g/g) and STC (0.237 g/g) but not OTA on black olives. These results indicate that STC, which is precursor of aflatoxin biosynthesis in A. flavus and A. parasiticus and also the end mycotoxin in secondary metabolism of other aspergilli, could be more frequently expected in olive oil than AFB1 and OTA. To our knowledge this is the first report on mycotoxin contamination of olive oil in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1242 - Mikromicete, interakcije toksičnih metabolita-zdravlje i prevencija (Maja Šegvić Klarić, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb