engleski

Stam2 expression pattern during embryo development

STAM2 is a tyrosine-phosphorylated protein suggested to be involved in cargo selection 3 during endocytic pathway, regulation of exocytosis and intracellular signaling. Gene trap 4 method was used to create via insertional mutagenesis a mutant mouse line with integration of 5 promoterless βgeo (lacZ-neomycin phosphotransferase fusion) gene in the second intron of 6 Stam2 gene, enabling analysis of its in vivo expression and function. The inserted β- 7 galactosidase (lacZ) reporter gene was used to reveal Stam2 expression during development. 8 Stam2 in situ RNA hybridization and immunostaining confirmed the observed β-galactosidase 9 activity reflecting high Stam2 expression. 10 The homozygous mutant mice showed no overt phenotypic alterations. Stam2 expression was 11 detected after E9.5 in the gut, notochord, neural tube and heart. In the nervous system it was 12 located in the floor, roof and basal plates of the developing neural tube, and in the developing 13 cortex, hippocampus and olfactory bulbs. Toward the end of gestation, Stam2 expression 14 appeared in the testis and ovary, lungs, nasal cavity epithelium, kidneys, urogenital sinus, 15 intestine, pancreas, pituitary and adrenal glands, muscles, brown adipose tissue, skin and 16 epithelium of the tongue and oral cavity.

STAM2; gene trap; mouse; embryo; expression; X-gal staining; development

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