Reinke's Crystals in Perivascular and Peritubular Leydig Cells (CROSBI ID 178210)
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Kozina, Viviana ; Banek, Ljerka ; Knežević , Nikola ; Geist , David ; Kubinova , Lucie ; Kosović , Marin ; Rentenberger , Christian ; Vukasović , Andreja ; Ježek , Davor
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Reinke's Crystals in Perivascular and Peritubular Leydig Cells
The human testis is composed of seminiferous tubules and interstitium. Within the interstitium, residing Leydig cells can occasionally bear Reinke´s crystals. The aim of the current study was to investigate Reinke´s crystals in perivascular and peritubular Leydig cells in control and infertile (cryptorchid) testes. For that purpose, bright field, confocal and transmission electron microscopy were applied. The crystal lattice was investigated by Fast Fourier Transformation and the number of crystals determined by stereology. Results of the study indicated a higher number of crystals in perivascular cells (in the both control and cryptorchid group). Moreover, when control and cryptorchid specimens were compared for the presence of the crystal, a higher number of Reinke´s crystal was recorded in cryptorchid testes. Thick sections of the crystal were extremely helpful in yielding crystallographic data which confirmed a trigonal crystal structure of the lattice. The exact molecular composition of crystal’s microfilaments still remains unknown.
biocrystals; crystal of Reinke; Leydig cells; transmission electron microscopy; crystallography
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Temeljne medicinske znanosti