engleski

Quantitative trait loci for metal accumulation into maize leaf

Maize as a major crop has been investigated for decades regarding metal accumulation, but not in the context of leaf ionome to identify putative genetic factors participating in the control of metal accumulation. Our objectives were to analyze variation for copper (Cu), iron (Fe) potassium (K), manganese (Mn), magnesium (Mg), strontium (Sr) concentrations in leaves of a maize mapping population, and to detect and to determine the effects of QTL associated with the metal concentrations. Ear-leaf samples at the beginning of the silking stage was taken for elemental analysis (ICP-OES) of 290 F4 lines of a biparental population (B84 x Os6-2) grown in field trials in Croatia. The population and parents differed significantly for Cu, Fe, K, Mg, Mn and Sr concentrations. The population was mapped using sets of 56 SNP and 65 SSR polymorphic markers. Eleven significant QTLs were detected for all six metal concentrations. Three of them, QTLs for Cu, Fe and Mg were colocalized on chromosome 5 in the region of ys1 gene. Significant dominant effect of these QTLs supports the involvement of ys1 in accumulations of these metals. Some QTLs had no obvious candidate genes offering the possibility of identifying unknown genes that affect metal accumulation.

metals ; maize ; leaf ; QTL

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