engleski

Development of a non-toxic, ecologically compatible, natural-resource based insecticide from diatomaceous earth deposits of South Eastern Europe to control stored-grain insect pests

Diatomaceous earth (DE) deposits from regions of Southeastern Europe were evaluated as regards their insecticidal effect against several insect pests that infest stored products such as S. oryzae (L.), Rhyzopertha dominica (F.), Tribolium confusum (DuVal), T. castaneum (Herbst), Cryptolestes ferrugineus (Steph.). Fifteen DE deposits were obtained from different geographical locations of Greece (Kriti, Elassona, Begora), Serbia (Kolubara, Vamje), Former Yugoslavic Republilc of Macedonia (F.Y.R.O.M.), Slovenia and Germany. Also, the commercially available DE formulation Silicosec was used as positive control. Initially the effect of temperature and r.h. on the efficacy of the local DEs was evaluated. The local DEs were applied on hard wheat at 900 ppm at three temperatures (20 °c, 25 °c and 30 °C) and two levels of relative humidity (55 ± 5% and 65 ± 5%). Mortality of adults of S. oryzae, R. dominica and T. confusum was evaluated after 7d of exposure of the individuals on the treated commodity. Next, the effect of grain type on the insecticidal action of them local DEs against adults of the above species was evaluated. In this series of experiments, the local DEs were applied at two dose rates (500 and 1000 ppm) at four different grain commodities ; bar1ey, hard wheat, maize and paddy rice. Mortality of the above species was assessed after 7d of exposure at 25 °c / 60 ± 5% r.h. Subsequently, fractions of the local DEs were obtained by dry sieving through different mesh sieves. Thus, for each local DE, three fractions with particles <45 microns, 45- 150 microns and 0-150 microns were obtained and each of them was applied on hard wheat at 1000ppm. Effectiveness of each fraction of the local DEs was assessed. Diatomaceous earth (DE) deposits from regions of Southeastern Europe were evaluated as regards their insecticidal effect against several insect pests that infest stored products such as S. oryzae (L.), Rhyzopertha dominica (F.), Tribolium confusum (DuVal), T. castaneum (Herbst), Cryptolestes ferrugineus (Steph.). Fifteen DE deposits were obtained from different geographical locations of Greece (Kriti, Elassona, Begora), Serbia (Kolubara, Vamje), Former Yugoslavic Republilc of Macedonia (F.Y.R.O.M.), Slovenia and Germany. Also, the commercially available DE formulation Silicosec was used as positive control. Initially the effect of temperature and r.h. on the efficacy of the local DEs was evaluated. The local DEs were applied on hard wheat at 900 ppm at three temperatures (20 °c, 25 °c and 30 °C) and two levels of relative humidity (55 ± 5% and 65 ± 5%). Mortality of adults of S. oryzae, R. dominica and T. confusum was evaluated after 7d of exposure of the individuals on the treated commodity. Next, the effect of grain type on the insecticidal action of them local DEs against adults of the above species was evaluated. In this series of experiments, the local DEs were applied at two dose rates (500 and 1000 ppm) at four different grain commodities ; bar1ey, hard wheat, maize and paddy rice. Mortality of the above species was assessed after 7d of exposure at 25 °c / 60 ± 5% r.h. Subsequently, fractions of the local DEs were obtained by dry sieving through different mesh sieves. Thus, for each local DE, three fractions with particles <45 microns, 45- 150 microns and 0-150 microns were obtained and each of them was applied on hard wheat at 1000ppm. Effectiveness of each fraction of the local DEs was assessed.

Diatomaceous earth ; deposits ; stored product pests ; control

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