engleski

Lack of Association of Interleukin-10 Polymorphism and Prostate Cancer Risk in Eastern Croatian Population

Introduction Recent studies suggest that chronic inflammation is crucial in the development and progression of prostate cancer (CaP). Inflammatory response depends upon differential production of cytokines, which is correlated with single- nucleotide polymorphisms (SNPs) of cytokine genes. So far, the studies of cytokine genes’ SNPs have shown contradictory results and propose ethnical dependency of SNPs. Interleukin-10 (IL10) has dual properties: it can reduce tumor angiogenesis, but can also enable tumor cells to «escape» the immune response with its anti-inflammatory action. We here investigated the association of two IL10 SNPs (IL10-819 C>T and IL10-592 C>A) with CaP risk in Eastern Croatian population. Patients and Methods 120 CaP patients and 120 benign prostatic hyperplasia (BPH) controls treated at the Clinic for Urology, University Hospital Centre Osijek were included in this study, after giving informed consent. Groups were age-matched. Two subgroups were formed according to Gleason score (≥4+3 and ≤3+4). To determine the IL10 SNPs, genomic DNA was extracted from 200 µl ethylenediaminetetraacetic acid (EDTA) whole blood using commercially available kits. IL10 gene polymorphism was assessed by real-time PCR method with melting curve analysis. Descriptive statistical analysis was performed with SPSS 19.0 statistical program (SPSS Inc., Chicago, Ill, USA) ; x2 test was used to determine the differences between the groups and Kendall’s tau-b test for linear-by-linear association. Results For the IL10-819 and -592 SNPs there were no significant differences between cases and controls. The -819 TT variant was associated with a 2-fold decreased risk for CaP, but the trend was not significant (OR = 0.5, 95% CI 0.16-1.54, p trend = 0.6). The -592 allele distribution was identical in cases and controls. When comparing the subgroups, the -819 TT variant showed a slightly increased risk for higher scores (OR = 1.23, 95% CI 0.19-7.84, p trend = 0.88). The two IL10 SNPs were also tested according to PSA levels (<10 and ≥10 ; >20 and ≥20) but again the groups did not differ. Discussion Although there are studies that report the association between the IL10 SNPs and CaP risk, recent meta-analysis of 10 studies on the subject found no significant association. Our analysis also shows lack of such an association for Eastern Croatian Population. These findings, taken together with contradictory results of other cytokine genes’ SNPs imply the ethnical dependency of such polymorphisms. Also, combinations and interactions of SNPs might have a greater impact on CaP risk than just one SNP. Future studies will define true importance of cytokine genes’ SNPs in the development and progression of CaP.

IL-10 ; polymorphism ; prostate cancer

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