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Pregled bibliografske jedinice broj: 548391

Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula


Wang, Xiaohong; Wiens, Matthias; Divekar, Mugdha; Grebenjuk, Vladislav A.; Schroeder, Heinz C.; Batel, Renato; Mueller, Werner E.G.
Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula // Marine drugs, 9 (2011), 1; 1-28 doi:10.3390/md9010001 (međunarodna recenzija, članak, znanstveni)


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Naslov
Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula

Autori
Wang, Xiaohong ; Wiens, Matthias ; Divekar, Mugdha ; Grebenjuk, Vladislav A. ; Schroeder, Heinz C. ; Batel, Renato ; Mueller, Werner E.G.

Izvornik
Marine drugs (1660-3397) 9 (2011), 1; 1-28

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Suberites domuncula ; Mn-precipitating bacteria ; Mn(II)-oxidizing enzyme

Sažetak
In this study we demonstrate that the demosponge Suberites domuncula harbors a Mn(II)-oxidizing bacterium, a Bacillus strain, termed BAC-SubDo-03. Our studies showed that Mn(II) stimulates bacterial growth and induces sporulation. Moreover, we show that these bacteria immobilize manganese on their cell surface. Comparison of the 16S rDNA sequence allowed the grouping of BAC-SubDo-03 to the Mn-precipitating bacteria. Analysis of the spore cell wall revealed that it contains an Mn(II)-oxidizing enzyme. Co-incubation studies of BAC-SubDo-03 with 100 mu M MnCl(2) and >1 mu M of CuCl(2) showed an increase in their Mn(II)-oxidizing capacity. In order to prove that a multicopper oxidase-like enzyme(s) (MCO) exists in the cell wall of the S. domuncula-associated BAC-SubDo-03 Bacillus strain, the gene encoding this enzyme was cloned (mnxG-SubDo-03). Sequence alignment of the deduced MCO protein (MnxG-SubDo-03) revealed that the sponge bacterium clusters together with known Mn(II)-oxidizing bacteria. The expression of the mnxG-SubDo-03 gene is under strong control of extracellular Mn(II). Based on these findings, we assume that BAC-SubDo-03 might serve as a Mn reserve in the sponge providing the animal with the capacity to detoxify Mn in the environment. Applying the in vitro primmorph cell culture system we could demonstrate that sponge cells, that were co-incubated with BAC-SubDo-03 in the presence of Mn(II), show an increased proliferation potential.

Izvorni jezik
Engleski

Znanstvena područja
Geologija



POVEZANOST RADA


Projekti:
098-0982705-2724 - Učinak zagađenja na programirane biosinteze morskih beskralješnjaka (Batel, Renato, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Renato Batel (autor)

Poveznice na cjeloviti tekst rada:

doi fulir.irb.hr doi.org

Citiraj ovu publikaciju:

Wang, Xiaohong; Wiens, Matthias; Divekar, Mugdha; Grebenjuk, Vladislav A.; Schroeder, Heinz C.; Batel, Renato; Mueller, Werner E.G.
Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula // Marine drugs, 9 (2011), 1; 1-28 doi:10.3390/md9010001 (međunarodna recenzija, članak, znanstveni)
Wang, X., Wiens, M., Divekar, M., Grebenjuk, V., Schroeder, H., Batel, R. & Mueller, W. (2011) Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula. Marine drugs, 9 (1), 1-28 doi:10.3390/md9010001.
@article{article, year = {2011}, pages = {1-28}, DOI = {10.3390/md9010001}, keywords = {Suberites domuncula, Mn-precipitating bacteria, Mn(II)-oxidizing enzyme}, journal = {Marine drugs}, doi = {10.3390/md9010001}, volume = {9}, number = {1}, issn = {1660-3397}, title = {Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula}, keyword = {Suberites domuncula, Mn-precipitating bacteria, Mn(II)-oxidizing enzyme} }
@article{article, year = {2011}, pages = {1-28}, DOI = {10.3390/md9010001}, keywords = {Suberites domuncula, Mn-precipitating bacteria, Mn(II)-oxidizing enzyme}, journal = {Marine drugs}, doi = {10.3390/md9010001}, volume = {9}, number = {1}, issn = {1660-3397}, title = {Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula}, keyword = {Suberites domuncula, Mn-precipitating bacteria, Mn(II)-oxidizing enzyme} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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