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Pregled bibliografske jedinice broj: 548164

Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells


Čipak, Ana; Borović, Suzana; Jaganjac, Morana; Bresgen, Nikolaus; Kirac, Iva; Grbeša, Ivana; Mrakovčić, Lidija; Cindrić, Marina; Šćukanec-Špoljar, Mira; Gall-Trošelj, Koraljka et al.
Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells // Acta biochimica Polonica, 57 (2010), 2; 185-191 (međunarodna recenzija, članak, znanstveni)


Naslov
Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells

Autori
Čipak, Ana ; Borović, Suzana ; Jaganjac, Morana ; Bresgen, Nikolaus ; Kirac, Iva ; Grbeša, Ivana ; Mrakovčić, Lidija ; Cindrić, Marina ; Šćukanec-Špoljar, Mira ; Gall-Trošelj, Koraljka ; Ćorić, Marijana ; Eckl, Peter ; Žarković, Neven

Izvornik
Acta biochimica Polonica (0001-527X) 57 (2010), 2; 185-191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
4-hydroxynonenal; spleen; hepatocyte; novel-liver derived cell line

Sažetak
Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a “physiological” factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
098-0982464-2511 - Epigenetičke i imunomodulatorne promjene u zloćudnim tumorima glave i vrata (Koraljka Gall-Trošelj, )
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )
108-0000000-0028 - Oksidacijski stres i tumori središnjeg živčanog sustava (Kamelija Žarković, )
108-0532264-0048 - Hepatocelularni tumori (Marijana Ćorić, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE