engleski

Effectivity of flavonoids in animal model of psoriasis

Psoriasis is a chronic inflammatory disease the immunopathogenesis, prevention and treatment of which have not been fully resolved. Through 5 days we investigated the potential beneficial effects of propolis-based substances and their poliphenolic/flavonoid compounds on an animal model psoriasis induced by the Di-n-Propyl Disulfide irritant (PPD). The inflammatory reaction was monitored by a termographic camera, by determining the overall number of cells in the peritoneal fluid, by measuring the hematologic and biochemical parameters in the blood and serum, and by a patohistologic analysis of skin samples. The possible genotoxic effects of stated substances were evaluated by comet and micronucleus tests. Results show that all the used propolis and/or flavonoid substances reduce the overall number of inflammatory cells in the peritoneal fluid of mice. Furthermore, the noticed changes in hematologic and biochemical parameters were well within the reference values, whereas the results of determining the cytokines in plasm showed that the PPD was, alone or mixed with stated substances, responsible for raised levels of inflammatory cytokines, especially the granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and when PPD was mixed with a water-soluble derivative of propolis (WSDP) or with quercetin, raised levels of interleukin-12 (IL-12) and interleukin-17 (IL-17) were noticed. Local application of flavonoids had lower G-CSF values in reference to the PPD and the combination of PPD and stated substances. The patohistologic skin analysis showed that combined local application of the irritant and used substances resulted in a much weaker inflammatory reaction than with PPD. The results of the comet and micronucleus tests show that propolis and poliphenols/flavonoids are not genotoxic for peripheral blood cells in mice. Finally, we can conclude that the used propolis and flavonoid substances show antiinflammatory properties, and that they could be used in preventing and treating inflammatory changes in psoriasis, without toxic side effects.

psoriasis; propolis; flavonoids

nije evidentirano