engleski

Effect of honey and quercetin on microflora digestive system and toxicity of Ochratoxin A in mouse

We investigated independent or combined effect of honey, quercetin and Lactobacillus reuteri to operate independently or combined effects of the toxicity caused by ochratoxin A in mice. We explore the link between biochemical, and immunological determinants hematopoietic and damage at the level of DNA, cells and organisms, and whether the food can improve activity of the intestinal microflora and inhibit the harmful and genotoxic effects of mycotoxins - link between diet and the number of colonies of intestinal microflora and the protective effect of honey, quercetin and lactobacilli from the consequences of OTA toxicity. Animals were divided into 10 groups of 7 mice according the treatment (control group, OTA, OTA + L, OTA + L + Q, OTA + L + honey, OTA + Q, OTA + MED, Q, MED, L), each second day through 15 days we used canulla for per os injection of: saline (0.9% NaCl), OTA (100 µg/kg) ; Lactobacillus reuteri (106 CFU per mouse), quercetin (50 mg/kg), honey (2 g/kg). We have noticed increase in the number of leukocytes in the group that received OTA + honey, as a result of increases in the number of active macrophages and lymphocytes. Stimulation of macrophages confirmed the protective and antioxidant role of honey, and reduced the effect of OTA metabolites. We also noticed increased number of erythrocytes and hematocrit in the group that received OTA + honey, quercetin and lactobacilli, but without statistical significance. Increased number of platelets in the group with honey confirmed the ability of honey in the regeneration of cells. At the biochemical determinants we noticed that the level of urea remained unchanged in the group who received the honey with OTA compared to control. Results obtained by counting colonies of probiotic bacteria column shows the largest number of colonies of mice, treatment with OTA + L. reuteri + quercetin. This result requires further study strains that formed a colony by PCR for the strain or other microbiological analysis. According to the obtained results, it appears that honey and quercetin probably slow down the deterioration of cells (apoptosis and/or necrosis) of blood, liver and kidney, while ochratoxin A causes rapid deterioration. Further evaluation of biological efficiency will significantly contribute to the protection of human health and animals frequently exposed to many harmful contaminants in food.

mycotoxins; protective effects; toxicity; ochratoxin

nije evidentirano