engleski

GENOTYPING OF TLR2 AND TLR4 GENES AND KIDNEY TRANSPLANTATION

Toll like receptors (TLRs) are crucial molecules for activation of adaptive immune system. We analyzed the distribution of TLR2 (T/C1350) and TLR4 (Asp299Gly) SNPs among 53 kidney transplanted patients, determined by Real time PCR with TaqMan probes. According to the obtained genotype patients were grouped into wild type or heterozygous group. Regarding the TLR4 gene, there were 5 heterozygous patients and 48 wild-types and for TLR2 gene there were 6 heterozygous and 47 wild type patients. Data on history of kidney function and immunosuppressive therapy were taken from medical records. Median time after transplantation was 8 years (min. 3, max. 24). Univariate analysis showed significant difference in serum urea concentration between wild-types and heterozygous patients for TLR2 gene and for TLR4 gene, respectively. Patients with wild type TLR2 and those with wild type TLR4 had higher urea concentrations, for TLR2 median 9.9, 3.8-58.3 mmol/l vs 6.2, 4.9-12 mmol/l ; z=-2.076, p=0.037, and for TLR 4 median 9.9, 3.8-58.3 mmol/l vs 5.5, 4.0-9.9 mmol/l ; z=-2.313, p=0.018 (Mann-Whitney test). Those TLR2 and TLR4 gene SNPs related differences in urea were not confirmed by multivariate analysis, when taken urea and time after transplantation as covariates, even though the patients with wild type TLR2 and TLR4 did not differ from heterozygous in the time after transplantation by univariate statistical analysis, respectively. Heterozygosity of the analyzed SNPs of TLR2 and TLR4 gene might still be protective regarding urea level in kidney transplanted patients. Further research in larger group is needed to confirm these findings.

dney transplantation; immune response; kidney function

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