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Pregled bibliografske jedinice broj: 545173

International round-robin study on the Ames fluctuation test


Reifferscheid, Georg; Maes, Hanna; Allner, Bernhard; Badurova, Jana; Belkin, Shimshon; Blum, Kerstin; Brauer, Frank; Bressling, Jana; Domeneghetti, Stefania; Elad, Tal et al.
International round-robin study on the Ames fluctuation test // Environmental and molecular mutagenesis, 53 (2012), 3; 185-197 doi:10.1002/em.21677 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 545173 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
International round-robin study on the Ames fluctuation test

Autori
Reifferscheid, Georg ; Maes, Hanna ; Allner, Bernhard ; Badurova, Jana ; Belkin, Shimshon ; Blum, Kerstin ; Brauer, Frank ; Bressling, Jana ; Domeneghetti, Stefania ; Elad, Tal ; Flückiger-Isler, Sini ; Grummt, Hans-Juergen ; Guertler, Rainer ; Hecht, Adiel ; Heringa, Minne ; Hollert, Henner ; Huber, Stefanie ; Kramer, Maike ; Magdeburg, Axel ; Ratte, Toni ; Sauerborn-Klobucar, Roberta ; Sokolowski, Andrea ; Soldan, Premsyl ; Smital, Tvrtko ; Stalter, Daniel ; Venier, Paola ; Ziemann, Christina ; Zipperle, Juergen ; Buchinger, Sebastian

Izvornik
Environmental and molecular mutagenesis (0893-6692) 53 (2012), 3; 185-197

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mutagenicity; genotoxicity; ISO; standardisation; round-robin study; Ames fluctuation test

Sažetak
An international round-robin study on the Ames fluctuation test (ISO 11350:2012), a microplate version of the classic plate-incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by eighteen laboratories from seven countries. Such a round-robin study is a pre-condition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. The laboratories tested four water samples (spiked/non-spiked) and two chemical mixtures with and without supplementation of a S9 mix. Validity criteria (acceptable spontaneous and positive control-induced mutation counts) were fulfilled by 92 to 100 %, depending on the test conditions. A two-step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine-square-root transformation in order to detect significant differences between the test samples and the negative control. A threshold value based on a pooled negative control was then calculated to exclude false positive test results. Statistically positive effects observed by the William’s test were considered negative, if the mean of all replicates of a sample did not exceed the calculated threshold. By making use of this approach the overall test sensitivity was 100 %, and the test specificity ranged from 80 to 100 %.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Smital, Tvrtko, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi onlinelibrary.wiley.com

Citiraj ovu publikaciju:

Reifferscheid, Georg; Maes, Hanna; Allner, Bernhard; Badurova, Jana; Belkin, Shimshon; Blum, Kerstin; Brauer, Frank; Bressling, Jana; Domeneghetti, Stefania; Elad, Tal et al.
International round-robin study on the Ames fluctuation test // Environmental and molecular mutagenesis, 53 (2012), 3; 185-197 doi:10.1002/em.21677 (međunarodna recenzija, članak, znanstveni)
Reifferscheid, G., Maes, H., Allner, B., Badurova, J., Belkin, S., Blum, K., Brauer, F., Bressling, J., Domeneghetti, S. & Elad, T. (2012) International round-robin study on the Ames fluctuation test. Environmental and molecular mutagenesis, 53 (3), 185-197 doi:10.1002/em.21677.
@article{article, author = {Reifferscheid, Georg and Maes, Hanna and Allner, Bernhard and Badurova, Jana and Belkin, Shimshon and Blum, Kerstin and Brauer, Frank and Bressling, Jana and Domeneghetti, Stefania and Elad, Tal and Fl\"{u}ckiger-Isler, Sini and Grummt, Hans-Juergen and Guertler, Rainer and Hecht, Adiel and Heringa, Minne and Hollert, Henner and Huber, Stefanie and Kramer, Maike and Magdeburg, Axel and Ratte, Toni and Sauerborn-Klobucar, Roberta and Sokolowski, Andrea and Soldan, Premsyl and Smital, Tvrtko and Stalter, Daniel and Venier, Paola and Ziemann, Christina and Zipperle, Juergen and Buchinger, Sebastian}, year = {2012}, pages = {185-197}, DOI = {10.1002/em.21677}, keywords = {mutagenicity, genotoxicity, ISO, standardisation, round-robin study, Ames fluctuation test}, journal = {Environmental and molecular mutagenesis}, doi = {10.1002/em.21677}, volume = {53}, number = {3}, issn = {0893-6692}, title = {International round-robin study on the Ames fluctuation test}, keyword = {mutagenicity, genotoxicity, ISO, standardisation, round-robin study, Ames fluctuation test} }
@article{article, author = {Reifferscheid, Georg and Maes, Hanna and Allner, Bernhard and Badurova, Jana and Belkin, Shimshon and Blum, Kerstin and Brauer, Frank and Bressling, Jana and Domeneghetti, Stefania and Elad, Tal and Fl\"{u}ckiger-Isler, Sini and Grummt, Hans-Juergen and Guertler, Rainer and Hecht, Adiel and Heringa, Minne and Hollert, Henner and Huber, Stefanie and Kramer, Maike and Magdeburg, Axel and Ratte, Toni and Sauerborn-Klobucar, Roberta and Sokolowski, Andrea and Soldan, Premsyl and Smital, Tvrtko and Stalter, Daniel and Venier, Paola and Ziemann, Christina and Zipperle, Juergen and Buchinger, Sebastian}, year = {2012}, pages = {185-197}, DOI = {10.1002/em.21677}, keywords = {mutagenicity, genotoxicity, ISO, standardisation, round-robin study, Ames fluctuation test}, journal = {Environmental and molecular mutagenesis}, doi = {10.1002/em.21677}, volume = {53}, number = {3}, issn = {0893-6692}, title = {International round-robin study on the Ames fluctuation test}, keyword = {mutagenicity, genotoxicity, ISO, standardisation, round-robin study, Ames fluctuation test} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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