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Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment


Reith, Lorenz M.; Schlagnitweit, Judith; Smrečki, Vilko; Knoer, Guenther; Mueller, Norbert; Schoefberger, Wolfgang
Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment // Magnetic resonance in chemistry, 49 (2011), 3; 125-128 doi:10.1002/mrc.2729 (međunarodna recenzija, članak, znanstveni)


Naslov
Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment

Autori
Reith, Lorenz M. ; Schlagnitweit, Judith ; Smrečki, Vilko ; Knoer, Guenther ; Mueller, Norbert ; Schoefberger, Wolfgang

Izvornik
Magnetic resonance in chemistry (0749-1581) 49 (2011), 3; 125-128

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NMR ; constant-time TOCSY experiment ; DNA oligomer ; 3J(1H3′-31P) coupling constants

Sažetak
A constant-time TOCSY difference experiment for the determination of 3J(1H3′-31P) coupling constants in non-isotope-labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant-time NOESY difference method. Each 3J(1H3′-31P) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross-peaks, thus leading to a high accuracy of the value of the 3J(1H3′-31P) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982929-2917 - Spektroskopija NMR i modeliranje bioaktivnih molekula (Dejan Plavšić, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Vilko Smrečki, (190003)

Citiraj ovu publikaciju

Reith, Lorenz M.; Schlagnitweit, Judith; Smrečki, Vilko; Knoer, Guenther; Mueller, Norbert; Schoefberger, Wolfgang
Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment // Magnetic resonance in chemistry, 49 (2011), 3; 125-128 doi:10.1002/mrc.2729 (međunarodna recenzija, članak, znanstveni)
Reith, L., Schlagnitweit, J., Smrečki, V., Knoer, G., Mueller, N. & Schoefberger, W. (2011) Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment. Magnetic resonance in chemistry, 49 (3), 125-128 doi:10.1002/mrc.2729.
@article{article, year = {2011}, pages = {125-128}, DOI = {10.1002/mrc.2729}, keywords = {NMR, constant-time TOCSY experiment, DNA oligomer, 3J(1H3′-31P) coupling constants}, journal = {Magnetic resonance in chemistry}, doi = {10.1002/mrc.2729}, volume = {49}, number = {3}, issn = {0749-1581}, title = {Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment}, keyword = {NMR, constant-time TOCSY experiment, DNA oligomer, 3J(1H3′-31P) coupling constants} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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