engleski

Epigenetic modulation of HeLa cell membrane N-glycome

Epigenetic changes play role in all major events during tumourogenesis and changes in glycan structures are hallmarks of virtually every cancer. Also, proper N-glycosylation of membrane receptors is important in cell to cell and cell-environment communication. To study modulation of epigenetic information can affect N-glycan expression we analysed effects of epigenetic inhibitors on HeLa cell membrane N-glycome. HeLa cells were treated with DNA methylation (zebularin and 5-aza-2-deoxycytidine) and histone deacetylation (trichostatin A and Na-butyrate) inhibitors. The effects on HeLa cell membrane N-glycome were analysed by hydrophilic interaction high performance liquid chromatography (HILIC). Each of the four epigenetic inhibitors induced changes in the expression of HeLa cell membrane N-glycans that were seen either as an increase or a decrease of individual glycans in the total N-glycome. Compared to DNA methylation inhibitors, histone deacetylation inhibitors showed more moderate changes, probably due to their higher gene target selectivity. The results clearly show that composition of HeLa cell membrane N- glycome can be specifically altered by epigenetic inhibitors. Glycans on the cell membrane are essential element of tumour cell’s metastatic potential and are also entry point for nearly all pathogenic microorganisms. Since epigenetic inhibitors used in this work are registered drugs, our results provide a new line of research in the application of these drugs as anticancer and antimicrobial agents.

DNA methylation; epigenetic inhibitors; HeLa cell line; histone acetylation; N-glycosylation; membrane N-glycome

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