Napredna pretraga

Pregled bibliografske jedinice broj: 541639

The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection


Benić, Miroslav; Habrun, Boris; Kompes, Gordan; Mihaljević, Željko; Cvetnić, Željko; Brstilo, Mate; Cergolj, Marijan; Maćešić, Nino
The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection // Milchwissenschaft, 67 (2012), 3; 296-299 (međunarodna recenzija, članak, znanstveni)


Naslov
The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection

Autori
Benić, Miroslav ; Habrun, Boris ; Kompes, Gordan ; Mihaljević, Željko ; Cvetnić, Željko ; Brstilo, Mate ; Cergolj, Marijan ; Maćešić, Nino

Izvornik
Milchwissenschaft (0026-3788) 67 (2012), 3; 296-299

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Milk; leukocytes; mastitis

Sažetak
Flow cytometry was applied in order to analyse cell content in udder secretions from cows infected by Streptococcus agalactiae (n=10), non- agalactiae streptococci (n=29) and uninfected udder quarters (n=7). The infection status of the quarters was checked three times with a 7-day gap interval between samplings. The somatic cell count was checked using Fossomatic 5000 basic. Cells were double labelled with anti-CD45 and one of the antibodies specific for granulocytes, CD4, CD8, B lymphocytes or monocytes and analysed by FacsCalibur. The somatic cell count, viability and percentage of labelled granulocytes differed significantly between the infected and uninfected quarters. The association of cell percents with management and physiological factors such as: origin of the animal, breed, age, gravidity, milking type, premilking and postmilking teat sanitation, grazing and vitamin/mineral feed supplements were checked using a univariate statistical analysis within each group. Age was associated with viability in the uninfected group, with a percentage of granulocytes in S. agalactiae and the non- agalactiae group. Gravidity was associated with viability in the uninfected group and CD8+ in the non-agalactiae group. Grazing was associated with CD4+ in both the infected groups and with B lymphocytes in the non-agalactiae group. The percentage of CD4+ and CD8+ lymphocytes differed significantly between hand and machine milked animals in the S. agalactiae group. The SCC, the percentage of granulocytes and monocytes varied significantly in regards to breed in the non- agalactiae group.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481186-1178 - Antimikrobna rezistencija bakterija značajnih u veterini (Boris Habrun, )
053-0532052-2044 - Program suzbijanja mastitisa (Goran Bačić, )

Ustanove
Hrvatski veterinarski institut, Zagreb,
Veterinarski fakultet, Zagreb

Citiraj ovu publikaciju

Benić, Miroslav; Habrun, Boris; Kompes, Gordan; Mihaljević, Željko; Cvetnić, Željko; Brstilo, Mate; Cergolj, Marijan; Maćešić, Nino
The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection // Milchwissenschaft, 67 (2012), 3; 296-299 (međunarodna recenzija, članak, znanstveni)
Benić, M., Habrun, B., Kompes, G., Mihaljević, Ž., Cvetnić, Ž., Brstilo, M., Cergolj, M. & Maćešić, N. (2012) The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection. Milchwissenschaft, 67 (3), 296-299.
@article{article, year = {2012}, pages = {296-299}, keywords = {milk, leukocytes, mastitis}, journal = {Milchwissenschaft}, volume = {67}, number = {3}, issn = {0026-3788}, title = {The association of milk cell subsets with management and physiological factors in cows with a natural streptococcal udder infection}, keyword = {milk, leukocytes, mastitis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus