engleski

Mucine 1 supports alternative activation of tissue specific decidual CD14+ cells with properties to restrict proliferation of NK cells poor with cytotoxic mediators

In order to better understand the reasons for MUC 1 disappearance at the uterine implantation site, we investigated the influence of mucin 1 (MUC1) on phenotype and function of early pregnancy decidual CD14+ cells and their interaction with cognate decidual NK cells. Flow cytometry was used for FITC-dextran internalisation, macrophage and NK cell markers detection and CD14+ cell mediated proliferation of CD56+ cells. Magnetic separation was used for CD56+ cells and CD14+ cells separation. Decidual CD14+ cells show lower CD16 expression, higher endocytic mannose receptors (MR) and dendritic cell specific intracellular adhesion molecule grabing nonintegrin (DC- SIGN), pro-inflammatory chemokine CC receptor 5 (CCR5) and decoy CD163 receptor expression, when compared with peripheral blood counterparts. MUC1 binds and internalises on a dose dependent manner carbohydrate recognition domain (CRD) of MR, but lypopolysaccharide stimulation did not affect FITC-dextran internalisation in decidual CD14+ cells. MUC 1 increases decoy IL-1 receptor type II and decreases frequency of IL-15 expressing CD14+ cells. In the presence of MUC1 treated macrophages, both the proliferation and cytotoxic mediators’ expression (perforin, Fas ligand and TNF related activation induced ligand or TRAIL) in NK cells attenuated, whereas anti-inflammatory chemokine CCL17 increased when compared with untreated macrophages. MUC1 supports alternative activation of tissue specific decidual CD14+ cells with a properties to restrict proliferation of NK cells poor with cytotoxic mediators. In accordance, MUC 1 removal from decidual tissue during and after implantation might contribute to better control of trophoblast invasion by NK cells.

alternative macrophage activation; CD14; cytotoxic mediators; mucine 1; NK cells; progesterone

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