engleski

Progesterone induced blocking factor (PIBF) downregulates CD91 heat shock receptor expression on decidual activated dendritic cells and NK cells.

Tissue remodeling in the first trimester human pregnancy decidua is associated with necrosis and death of various cells followed by the heat shock protein (HSP) release in the extra cellular space, where they become forceful immunogens after the binding with cognate receptors on immune cells. Previously we found the expression of HSP 70 in decidual tissue and its cognate CD91 receptor expression on decidual cells. It implies the presence of molecular basis for the strong immune response which fortunately does not occur during normal pregnancy. In this study we investigated whether mediator of progestetrone action named progesterone induced blocking factor (PIBF) affects the expression of CD91 receptor on the cells of innate immunity. The setting of decidual CD56+ cells, CD14+ macrophages, CD1a+ and CD83+ dendritic cells, HSP70+ and PIBF+ cells were investigated by immunohistology of the first trimester decidual tissue sections. Decidual mononuclear cells were isolated from early decidual tissue by collagenase IV digestion and gradient density centrifugation and stimulated overnight with increasing concentrations of PIBF. The expression of CD91 was analyzed by triple color flow cytometry. Decidual glandular epithelial cells show surface and cytoplasmic labeling with anti-HSP 70 mAb. CD56+ cells, CD14+ macrophages, CD1a+ and CD83+ dendritic cells were situated around the glands enabling the interaction with glandular products. Strong cytoplasmic PIBF+ labeling was found in the cells spreaded widely throughout the stroma. PIBF, after the stimulation in vitro, significantly down regulated CD91 receptor expression in decidual CD3-CD56dim+ NK cells and mature CD83+ dendritic cells, whereas CD3-CD56bright+ NK cells, CD1a+ dendritic cells and CD14+ macrophages remained basically unchanged. PIBF down-regulates CD91 expression on activated decidual cells of innate immunity controlling that way the initiation and of the immune response in normal early pregnancy decidua.

CD91; heat shock protein; immune cells; pregnancy; progesterone

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