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Pregled bibliografske jedinice broj: 540975

Changes in frequency of peripheral blood lymphocyte subpopulations in patients with myocardial infarction during a period of early medical rehabilitation


Raljević, Damir; Peršić, Viktor; Ružić, Alen; Miletić, Bojan; Travica Samsa, Dijana; Rakić, Marijana; Pehar Pejčinović, Vesna; Laškarin, Gordana
Changes in frequency of peripheral blood lymphocyte subpopulations in patients with myocardial infarction during a period of early medical rehabilitation // Electronic abstract book
Prag, Češka, 2010. str. Abstract 521-Abstract 521 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Changes in frequency of peripheral blood lymphocyte subpopulations in patients with myocardial infarction during a period of early medical rehabilitation

Autori
Raljević, Damir ; Peršić, Viktor ; Ružić, Alen ; Miletić, Bojan ; Travica Samsa, Dijana ; Rakić, Marijana ; Pehar Pejčinović, Vesna ; Laškarin, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Electronic abstract book / - , 2010, Abstract 521-Abstract 521

Skup
EuroPRevent 2010

Mjesto i datum
Prag, Češka, 07.-10. 05. 2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lymphocyte subpopulation; myocardial infarction; peripheral blood

Sažetak
Purpose: The site of infarction and remote myocardial regions are characterized with inflammatory reaction and leukocyte infiltration. However, there is insufficient knowledge about the involvement of peripheral blood lymphocyte subpopulations in this inflammatory process. The purpose of the study was to investigate changes in the frequency of lymphocyte subpopulations in peripheral blood of patients with myocardial infarctation (MI) during the early medical rehabilitation period. Methods: Twelve patients with ST elevation MI (STEMI) and diminished possibility for further progression of the coronary artery disease due to percutaneous coronary intervention and 12 patients with non-STEMI who were treated with standard drug therapy were enrolled in this pilot study. The group of twenty healthy volunteers was used as the control. Peripheral blood samples were taken from MI patients on day 7, 14, 21 and 28 after the acute coronary event and peripheral blood mononuclear cells (PBMC) were immediately isolated by gradient density centrifugation. The frequencies of lymphocyte subpopulation were analyzed by simultaneous assessment of surface markers using fluorochrome conjugated monoclonal antibodies directed toward CD3 and CD56 molecules. T cells were distinguished from the other lymphocyte subpopulations as cells labeled with anti-CD3 monoclonal antibody, but negative for CD56 staining (CD3+CD56- cells). The cells stained as CD3-CD56+ were determined as NK cells, whereas CD3+ and CD56+ cells were counted as NK/T cells. Results: In patients with non-STEMI the frequency of T cells decreased on day 7 and 14 and in the patients with STEMI on day 14 and 21 after the acute myocardial ischemia in comparison with healthy persons. At the end of early rehabilitation period on day 28 the percentage of T cells is restored in both groups of patients. The frequency of NKT cells in peripheral blood of patients with non-STEMI increased on day 14 when compared with healthy volunteers and patients with STEMI. On day 21 and 28 the percentage of NKT cells did not differ from the control. The frequency of NK cells did not significantly change at time points investigated. Conclusions: The fluctuation of peripheral blood T and NKT cells during early medical rehabilitation period suggests their participation in myocardial remodeling events. The investigation of their activation status would provide better insight in immune response and it would open possibilities for new approaches in early medical rehabilitation of patients with MI.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0620402-0377 - Imunoregulacijske funkcije antigen predočnih stanica tijekom rane trudnoće (Gordana Laškarin, )

Ustanove
Medicinski fakultet, Rijeka