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Pregled bibliografske jedinice broj: 540761

Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum


Mosoarca, Cristina; Ghiulai, Roxana M.; Novaconi, Cristina R.; Vukelić, Željka; Chiriac, Adrian; Zamfir, Alina D.
Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum // Analytical letters, 44 (2011), 6; 1036-1049 doi:10.1080/00032719.2010.506938 (međunarodna recenzija, članak, znanstveni)


Naslov
Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum

Autori
Mosoarca, Cristina ; Ghiulai, Roxana M. ; Novaconi, Cristina R. ; Vukelić, Željka ; Chiriac, Adrian ; Zamfir, Alina D.

Izvornik
Analytical letters (0003-2719) 44 (2011), 6; 1036-1049

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Automation; cerebellum; chip-nanoelectrospray; gangliosides; ion trap mass spectrometry; NanoMate

Sažetak
Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15)and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS2)analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS2 molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Željka Vukelić, )

Ustanove
Medicinski fakultet, Zagreb

Autor s matičnim brojem:
Željka Vukelić, (241834)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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