engleski

The time course of kidney lesions in ochratoxin A treatment

Ochratoxin A (OTA) is nephrotoxic, genotoxic and carcinogen mycotoxin that contaminates food in all climatic conditions. The mechanism of its toxicity is not fully understood. In our studies male adult Wistar rats were treated orally with 0, 0.125 and 0.250 mg OTA/kg b.w. (dissolved in NaHCO3), respectively, daily for 21 days. Before the beginning of the experiment and after 3, 6, 9, 12, 15, 18, and 21 treatments animals were kept for 24 hours in metabolic cages for collecting urine. In urine, malodialdehyde (MDA) as a parameter of lipid peroxidation in whole organism was measured using HPLC method with UV detector. The concentration of glucose using spectrophotometic method was also measured in urine. At both doses the concentration of MDA increased gradually until the 15th treatment and then decreased albeit the continuation of OTA treatment. Mean±SD concentration of MDA in animals treated with 0, 0.125 and 0.250 mg OTA/kg b.w. was 2.08±0.42, 2.36±0.92 and 3.80±0.60 µmol/L, respectively. At this time-point MDA concentration in animals treated with 0.250 mg OTA was significantly higher than in controls (P<0.05). The peak of the glucose concentration in urine appeared earlier, after the 6th OTA treatment (0.98±0.58, 0.90±0.42, and 3.4±0.70 mmol/L, respectively) and then started to decrease. After the 6th treatment the glucose concentration was significantly higher in urine of animals treated with 0.250 mg OTA then in controls (P<0.05). In animals sacrificed 24 hours after 21 treatments blood, kidney and liver were collected. In these tissues MDA and glutathione (GSH) as measures of local oxidative stress were determined. OTA treatment did not affect the concentration of MDA in all analyzed tissues. The GSH concentration in all tissues showed decreasing pattern with the increase of OTA dose. Our results indicate that kidney may recover from the oxidative lesions caused by OTA.

ochratoxin A; malondialdehyde; glutathione

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