engleski

Sensing of aquatic pollutants: mixed lipid monolayers as a sensing template

The importance of oceans and waters in general and the need to monitor and exploit them increases the necessity for fast sensors for pollutants. Lipophylic aquatic pollutants show biomembrane activity [1]. The interaction causes a disruption of the lipid monolayer organization, which can be monitored using alternating current (ac) and fast cyclic (cv) voltammetry on a hanging mercury drop electrode (HMDE). Development of a biomembrane mimicking electrochemical biosensor using mixed lipid monolayers optimised for interactions with specific analyte will be discussed. Mixed monolayers of palmitoyl-2-oleoyl-sn-glicero-3- phosphocholin (POPC) and triolein, a trigliceride of oleic acid, were tested in interactions with phenanthrene. In order to improve the specificity and sensitivity of the monolayer, different molar ratios of lipids were tested. Figure 1. shows a shift of the reorientation peak potential of monolayer of POPC and triolein in a 3:1 molar ratio with increasing concentrations of phenanthrene. The work was also done using a flow cell system with a chip based mercury microelectrode [2]. Further optimisation of the mixed lipid monolayers will be made in order to enable detection of different type of pollutants such as polychlorinated biphenyls, tributyltins and toxins such as domoic and ocadaic acid.

Sensor; pollutants; mixed lipid monolayers

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