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Pregled bibliografske jedinice broj: 538280

IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774


Gabrilovac, Jelka; Čupić, Barbara; Zapletal, Emilija; Brozović, Anamaria
IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774 // Journal of neuroimmunology, 245 (2012), 1-2; 56-65 doi:10.1016/j.jneuroim.2012.02.006 (međunarodna recenzija, članak, znanstveni)


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Naslov
IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774

Autori
Gabrilovac, Jelka ; Čupić, Barbara ; Zapletal, Emilija ; Brozović, Anamaria

Izvornik
Journal of neuroimmunology (0165-5728) 245 (2012), 1-2; 56-65

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
kappa opioid receptors (KOR) ; IFN-gamma ; J774 cells ; macrophages ; transcriptional expression ; protein expression

Sažetak
In this study we examined basal and IFN-γ-regulated expression of kappa opioid receptors (KOR) on cells of a murine macrophage cell line, J774. Basal KOR expression was found both at transcriptional and protein levels. KOR protein was predominantly located intracellular (86.4 ± 12.9% positive cells ; n = 4), and only in minority of J774 cells (9.1 ± 6.4% positive cells ; n = 4) on plasma membranes, as revealed by Fluorescence-Activated Cell Sorter (FACS) analysis and immunocytochemistry. Proinflammatory cytokine IFN-γ up-regulated KOR expression both at transcriptional (up to 24 times) and protein levels (up to 4.2 times). KOR expressed on J774 cells was functionally active as its ligation with Dynorphin-A(1–17) (100 nM and 1 μM) triggered phosphorylation of ERK1/2. Involvement of KOR in the Dynorphin-A(1–17)-induced triggering of ERK1/2 phosphorylation is suggested since truncated Dynorphin-A(2–17), which does not bind to KOR, was ineffective. Collectively, we have shown for the first time that cells of J774 cell line constitutively express functionally active KOR, which triggers signalization via ERK1/2 phosphorylation and which could be up-regulated by proinflammatory IFN-γ. The data may be relevant for better understanding of the role of KOR and their endogenous ligand Dynorphin-A in regulation of inflammatory and immune responses.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2520 - ULOGA MEMBRANSKIH PEPTIDAZA NA TUMORSKIM I NORMALNIM STANICAMA (Jelka Gabrilovac, )
098-0982913-2748 - Stanični odgovor na citotoksične spojeve i razvoj otpornosti (Maja Osmak, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Gabrilovac, Jelka; Čupić, Barbara; Zapletal, Emilija; Brozović, Anamaria
IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774 // Journal of neuroimmunology, 245 (2012), 1-2; 56-65 doi:10.1016/j.jneuroim.2012.02.006 (međunarodna recenzija, članak, znanstveni)
Gabrilovac, J., Čupić, B., Zapletal, E. & Brozović, A. (2012) IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774. Journal of neuroimmunology, 245 (1-2), 56-65 doi:10.1016/j.jneuroim.2012.02.006.
@article{article, year = {2012}, pages = {56-65}, DOI = {10.1016/j.jneuroim.2012.02.006}, keywords = {kappa opioid receptors (KOR), IFN-gamma, J774 cells, macrophages, transcriptional expression, protein expression}, journal = {Journal of neuroimmunology}, doi = {10.1016/j.jneuroim.2012.02.006}, volume = {245}, number = {1-2}, issn = {0165-5728}, title = {IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774}, keyword = {kappa opioid receptors (KOR), IFN-gamma, J774 cells, macrophages, transcriptional expression, protein expression} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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