engleski

Renal osteodistrophy in chronic renal disease in rat

The aim of the present study was the evaluation of receptor activator of nuclear factor-kappaB ligand RANKL in concern with bone serum markers in rats with induced end stage renal disease (ESRD) and correlation with bone histomorphometric changes. The rats undergone 5/6 nephrectomy and three months later were sacrificed. We measured serum levels of intact PTH (iPTH), osteocalcin (OC), and tartrate-resistant acid phosphatase (TRAP) in 30 ESRD rats and in 15 healthy controls. Bone histomorphometry on femur was determined by micro- CT. Bones were decalcified in 10% EDTA solution, embedded in paraffin and immunohistochemistry was performed. We determined in bone tissue alkaline phosphatase and TRAP enzyme activity as well as the RANKL expression. The average serum OC level was lower, whereas the average serum concentration of TRAP was higher in ESRD rats than in healthy controls. ESRD in all rats did not affect increase of iPTH. The expression of RANKL did not correlate with iPTH levels. The alkaline phosphatase and TRAP positive cells in the bone marrow and trabecular surface were decreased in ESRD rats. The RANKLexpression decreased also in bone marrow stromal osteoblasts. All bone histomorphometric parameters confirmed loss of bone tissue.

renal osteodistrophy; chronic renal disease; RANKL; PTH; osteocalcin

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