engleski

CONSEQUENCES OF LOW-INTENSITY RF RADIATION ON THE INTRACELLULAR MACROMOLECULAR STRUCTURES

The option that radiofrequency (RF) radiation might induce undesired alterations at cellular level is a general scientific and public concern. The reason is extensive and growing development of different radiation resources including equipment for telecommunications and broadcasting systems, domestic devices, and medical apparatus. This study was carried out on rationale to evaluate the biological consequence of low-intensity RF radiation on intracellular macromolecular structures ; DNA and cytoskeleton protein network. The study was performed both in vivo and in vitro ; in particular the experimental animals and continuous culture of cells were used. In vivo experimental design incorporated exposed and unexposed control rats. Animal groups were divided into the subgroups in order to be sacrificed on 2, 8, 15 and 30 experimental day after irradiation daily treatment of two hours each. Rats were irradiated with 2450 MHz RF waves and an average power density of 7.5 mW/cm2, SAR=1.25 W/kg. In vitro design included continuous cell culture of lung fibroblasts exposed to a 915 MHz RF field and 10 V/m electric field strength, SAR=0.2 W/kg. Exposure lasted 1 h, 2 h, and 3 h. Micronucleus test (MN) on polychromatic erythrocytes (PCE) in vivo was performed. The same test was applied on in vitro system. Indirect immunocytochemical method was used to determine protein structure of microtubule and vimentin, which is a member of the intermediate filaments. Actin proteins were labeled with phalloidin-rhodamine complex. One thousand cells per slide were analyzed using a fluorescent light microscope (400 x magn). The MN test showed significantly more PCE-s with micronuclei in rats irradiated for 16 h that is on 8th day of experiment. The in vitro study confirmed the significantly higher occurrence of micronucleated cells as well as damage to polar microtubules and actin filaments, which correlated with the duration of radiation exposure. The intermediate filaments remained unaffected. Our in vivo and in vitro results confirm the possibility of unfavorable effects of low-intensity, radiofrequency radiation on intracellular macromolecular structures.

in vitro; in vivo; cytoskeleton; DNA

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