engleski

The role of cytoskeleton filaments polarity in the interaction with external radiofrequency field

The study was carried out on the purpose to get insight into the response of cytoskeleton filaments of different polarity after external radiofrequency (RF) radiation. Polar and non-polar cytoskeleton network filaments were observed after modulated RF radiation. Continuous V79 cell line was exposed to modulated RF radiation frequency of 915 MHz, electric field strength of 10, 20 and 30 V/m. Average specific absorption rate (SAR) was calculated at 0.23, 0.8 and 1.6 W/kg. Cell exposure treatment lasted for 1, 2, and 3 hours. Experimental cell groups were matched with negative- and positive control groups. Exposure set-up consisted Gigahertz Transversal Electromagnetic Mode chamber (GTEM), generator, power amplifier and signal modulator. Rhodamine-phalloidin fluorescent technique was used to mark polar actin microfilament structure. Cytoskeleton vimentin filaments were determined by indirect immunocytochemistry method. Both actin and vimentin structures were inspected within the five hundred cells which were observed under the fluorescent microscope, respectively. In comparison with negative control cell samples, actin microfilaments were significantly altered after three hour of exposure to 30 V/m electric field strength. Otherwise, vimentin filaments did not respond to applied radiation in any other way, i.e., it’s remained unchanged throughout the course of experiment. In conclusion: Contrary to non-polar cytoskeleton vimentin filaments, external modulated RF radiation frequency of 915 MHz might significantly damage the polar actin filament structures.

vimentin filaments; actin microfilament; Gigahertz Transversal Electromagnetic Mode chamber; microtubules

nije evidentirano