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Recombinatorial biosynthesis of polyketides


Starčević, Antonio; Wolf, Kerstin; Diminić, Janko; Žučko, Jurica; Long, F Paul; Cullum, John; Hranueli, Daslav
Recombinatorial biosynthesis of polyketides // Journal of industrial microbiology & biotechnology, 39 (2012), 3; 503-511 doi:10.1007/s10295-011-1049-x (međunarodna recenzija, članak, znanstveni)


Naslov
Recombinatorial biosynthesis of polyketides

Autori
Starčević, Antonio ; Wolf, Kerstin ; Diminić, Janko ; Žučko, Jurica ; Long, F Paul ; Cullum, John ; Hranueli, Daslav

Izvornik
Journal of industrial microbiology & biotechnology (1367-5435) 39 (2012), 3; 503-511

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Polyketides – Actinobacteria – gene clusters – homologous recombination

Sažetak
Modular polyketide synthases (PKSs) from Streptomyces and related genera of bacteria produce many important pharmaceuticals. A program called CompGen was developed to carry out in silico homologous recombination between gene clusters encoding PKSs and determine whether recombinants have cluster architectures compatible with the production of polyketides. The chemical structure of recombinant polyketides was also predicted. In silico recombination was carried out for 47 well-characterised clusters. The predicted recombinants would produce 11, 796 different polyketide structures. The molecular weights and average degree of reduction of the chemical structures are dispersed around the parental structures indicating that they are likely to include pharmaceutically interesting compounds. The details of the recombinants and the chemical structures were entered in a database called r-CSDB. The virtual compound library is a useful resource for computer-aided drug design and chemoinformatics strategies for finding pharmaceutically relevant chemical entities. A strategy to construct recombinant Streptomyces strains to produce these polyketides is described and the critical steps of mobilizing large biosynthetic clusters and producing new linear cloning vectors are illustrated by experimental data.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0000000-3475 - Generiranje potencijalnih lijekova u uvjetima in silico (Daslav Hranueli, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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