engleski

Expression of pannexin family of gap junction proteins in sporadic cardiac myxoma cases

Cardiac myxoma (CM) represents the most prevalent type of primary cardiac tumors in adults, constituting up to 50-85% of all benign lesions of the heart. Due to their strategic localization and inherent pathohistological characteristics, they are considered as quite malignant entities with very serious consequences for morbidity and mortality of affected patients. Surgical resection of cardiac myxoma is currently the only treatment of choice. The purpose of this study was to determine the expression pattern of pannexin family (Panx 1/2/3) of gap junction proteins in sporadic cardiac myxoma cases. A total of 28 consecutive patients with sporadic cardiac myxoma diagnosed from 2002 to 2010 at Department of Cardiac Surgery, University Hospital Centre Zagreb were included in the study. Tumour tissue sections obtained from archival FFPE tumor tissue blocks (n=25) or fresh surgical tissue samples (n=3) snap-frozen in liquid N2 were used for immunohistological staining and RNA isolation. Immunohistochemical staining was performed with rabbit polyclonal anti-human pannexin-1, 2 and 3 antibody (AbCam ab78380, ab55917, ab98093) using DAB substrate and HRP reaction. RNA isolation, in vitro transcription and qRT-PCR were performed using TRI Reagent and RNeasy Mini kit, gene specific Quanti Tect Primer Assay and 7500 qRT-PCR system. Obtained results showed positive expression pattern for Panx 1- 3 in tumor cells (lepidic tumor cells arranged singly or in small clusters and vasiforme cell aggregations) of all analyzed samples on protein and mRNA level. The significance, of pannexin gene expression as a marker for cardiac myxoma has still to be elucidated.

sporadic cardiac myxoma; pannexin 1; pannexin 2; pannexin 3; gap

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