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Pregled bibliografske jedinice broj: 534008

Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva


Brkljačić, Lidija; Sabalić, Maja; Salarić, Ivan; Jerić, Ivanka; Alajbeg, Ivan; Nemet, Ina
Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva // Journal of chromatography. B, 879 (2011), 32; 3920-3926 doi:10.1016/j.jchromb.2011.11.003 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 534008 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva

Autori
Brkljačić, Lidija ; Sabalić, Maja ; Salarić, Ivan ; Jerić, Ivanka ; Alajbeg, Ivan ; Nemet, Ina

Izvornik
Journal of chromatography. B (1570-0232) 879 (2011), 32; 3920-3926

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
opiorphin ; saliva ; LC-MS/MS ; biomarker

Sažetak
Opiorphin, QRFSR-peptide, is a mature product of the PROL1 (proline rich, lacrimal 1) protein that showed beneficial effects in pain management, antidepressant-like actions as well as involvement in colonic motility and erectile physiology. Using opiorphin as a potential biomarker of different pathological states requires the development of robust and sensitive methods. We report a highly sensitive and specific liquid chromatography with tandem mass spectrometric detection (LC– MS/MS) analytical method for the analysis of opiorphin in human saliva. Quantification was based on multiple reaction monitoring using characteristic transitions (m/z 347/120 – as quantifying ion ; 347/175 and 347/268 as qualifying ions). The assay was linear in the range of 0–110 ng/ml and the lower limit of quantification reached was 1.0 ng/ml. The intra-day precision and accuracy were between 2.7–5.6% and −2.3 to 3.2%, respectively. The inter-day precision and accuracy were between 10.8–13.7% and −11.0 to 52%, respectively. Mean recovery was 106% and mean matrix effect was 0.97. Opiorphin in TFA treated saliva samples was stable for at least 12 h at room temperature and up to 30 days at −20 ◦C. Opiorphin levels in human saliva samples collected from young healthy individuals ranged from 2.8 to 25.9 ng/ml.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Dentalna medicina



POVEZANOST RADA


Projekti:
065-0650445-1277 - Nearomatski naftalan –istraživanje sastava i bioloških učinaka na epitelna tkiva (Alajbeg, Ivan, MZOS ) ( POIROT)
098-0982933-2936 - Kemijske preobrazbe prirodnih spojeva (Varga-Defterdarović, Lidija, MZOS ) ( POIROT)

Ustanove:
Stomatološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Ina Nemet (autor)

Avatar Url Ivan Alajbeg (autor)

Avatar Url Ivanka Jerić (autor)

Avatar Url Ivan Salarić (autor)

Citiraj ovu publikaciju

Brkljačić, Lidija; Sabalić, Maja; Salarić, Ivan; Jerić, Ivanka; Alajbeg, Ivan; Nemet, Ina
Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva // Journal of chromatography. B, 879 (2011), 32; 3920-3926 doi:10.1016/j.jchromb.2011.11.003 (međunarodna recenzija, članak, znanstveni)
Brkljačić, L., Sabalić, M., Salarić, I., Jerić, I., Alajbeg, I. & Nemet, I. (2011) Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva. Journal of chromatography. B, 879 (32), 3920-3926 doi:10.1016/j.jchromb.2011.11.003.
@article{article, year = {2011}, pages = {3920-3926}, DOI = {10.1016/j.jchromb.2011.11.003}, keywords = {opiorphin, saliva, LC-MS/MS, biomarker}, journal = {Journal of chromatography. B}, doi = {10.1016/j.jchromb.2011.11.003}, volume = {879}, number = {32}, issn = {1570-0232}, title = {Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva}, keyword = {opiorphin, saliva, LC-MS/MS, biomarker} }
@article{article, year = {2011}, pages = {3920-3926}, DOI = {10.1016/j.jchromb.2011.11.003}, keywords = {opiorphin, saliva, LC-MS/MS, biomarker}, journal = {Journal of chromatography. B}, doi = {10.1016/j.jchromb.2011.11.003}, volume = {879}, number = {32}, issn = {1570-0232}, title = {Development and validation of a liquid chromatography – tandem mass spectrometry method for the quantification of opiorphin in human saliva}, keyword = {opiorphin, saliva, LC-MS/MS, biomarker} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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