engleski

Aetiology of increased micronucleus frequency in mononuclear lymphocytes and reticulocytes in newborns

Micronucleus frequencies in children are described to be significantly lower than in their mothers due to short time of environental exposure, absence of elimination of X or Y chromosomes and absence of genome instability. our recent study of 92 mothers (Rhea cohort)and their newborns using the MN assay, within the NewGeneris project, showed MN frequency in binuclear (BN) lymphocytes to be lower in the newborns than in the mothers and within the expected ranges for general population. MN frequency in mononuclear (MONO)lymphocytes in newborns was higher than in their mothers (2.05‰ vs. 1.34‰, respectively). Increased MN frequency in MONO cells was not related to the smoking habit of the mothers. Similar results og higher MN frequencies in MONO lymphocytes were shown in patients 7 years after radiotherapy (2.55‰ vs. 0.74‰ in control). the origin of these findings could be in disturbed availability of CD4 or CD 45 T lymphocyte for mitotic stimulation by phytohaemagglutinin. Using in vivo MN assay, increased MN frequencies in reticulocytes were detected in newborns of mothers who were smoking or taking antiepileptic drugs during pregnancy (3.14‰ and 3.09‰, respectively) versus 1.56‰ in newborns of nonsmoking mothers while all mothers had similar MN frequency (Fucic et al. 2010). these findings show significance of insight in immunologic status of subjects in interpretation of MN frequency and different age related sensitivity of cell types used for biomonitoring.

micronucleus assay; mononuclear lymphocytes; newborns

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