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Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin


Leščić Ašler, Ivana; Pigac, Jasenka; Vujaklija, Dušica; Luić, Marija; Štefanić, Zoran
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin // Acta crystallographica section f-structural biology and crystallization communications, 67 (2011), 11; 1378-1381 doi:10.1107/S1744309111032222 (međunarodna recenzija, članak, znanstveni)


Naslov
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin

Autori
Leščić Ašler, Ivana ; Pigac, Jasenka ; Vujaklija, Dušica ; Luić, Marija ; Štefanić, Zoran

Izvornik
Acta crystallographica section f-structural biology and crystallization communications (1744-3091) 67 (2011), 11; 1378-1381

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Streptomyces rimosus ; SGNH hydrolases ; lipases ; 3 ; 4-dichloroisocoumarin

Sažetak
A recombinant lipase (triacylglycerol acylhydrolase ; EC 3.1.1.3) from the bacterium Streptomyces rimosus was inhibited by the serine protease inhibitor 3, 4-dichloroisocoumarin and crystallized by the hanging-drop vapour-diffusion method at 291 K. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 38.1, b = 78.7, c = 56.6 Å, [beta] = 104.5° and probably two molecules in the asymmetric unit. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation on the XRD beamline of the Elettra synchrotron, Trieste, Italy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
F logocrystallization communications share



POVEZANOST RADA


Projekt / tema
098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Dušica Vujaklija, )
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Marija Luić, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • CA Search (Chemical Abstracts)
  • MEDLINE
  • Biochemistry and Biophysics Citation Index
  • Nucleic Acid Database
  • Protein Data Bank
  • Scopus


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