engleski

Malassezia pachydermatis in isolates from bears and wolves

The genus Malassezia can be found on the skin and mucous of different animal species. At present dogs and cats represent the best explored group of hosts of this lipophilic yeast. On the contrary, the wild animals as carriers of Malassezia species are much less examined. This study is undertaken to isolate and characterize Malassezia species from wild animals applying traditional microbiological and molecular methods. The swabs were taken from the left and right external ear canal, and anus of the brown bears and wolves and cultured on modified Dixon agar. Grown colonies of Malassezia pachydermatis were identified based on the microscopic morphology, and subcultured on Sabouraud dextrose agar in order to confirm its non lipid dependency. Thirteen sequences from M. pachydermatis isolates were analyzed on the basis of the first internal transcribed spacer (ITS-1). DNA was isolated with Invitrogen PureLinkTM Genomic DNA mini kit. PCR products amplified with specific primers 18 SF1 (5'-AGGTTTCCGTAGGTGAACCT-3') and 5.8 SR1 (5'-TTCGCTGCGTTCTTCATCGA-3') corresponded to a 282 region that of ITS-1 sequence. Sequence analysis showed molecular homogeneity, and all isolates analyzed so far, were identified as M. pachydermatis.

Malassezia pachydermatis; bears; wolves;

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