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Pregled bibliografske jedinice broj: 529767

Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases


Močibob, Marko; Ivić, Nives; Šubašić, Deni; Luić, Marija; Weygand-Đurašević, Ivana
Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases // Croatica chemica acta, 84 (2011), 2; 149-157 doi:10.5562/cca1818 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 529767 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases

Autori
Močibob, Marko ; Ivić, Nives ; Šubašić, Deni ; Luić, Marija ; Weygand-Đurašević, Ivana

Izvornik
Croatica chemica acta (0011-1643) 84 (2011), 2; 149-157

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aminoacyl-tRNA synthetase; amino acid:[carrier protein] ligase; carrier protein; coenzyme A; seryl-tRNA synthetase; thioester; tRNA

Sažetak
Amino acid:[carrier protein] ligases (aa:CP ligases) are newly discovered homologs of aminoacyl-tRNA synthetases (aaRS) which aminoacylate carrier proteins instead of tRNA. Activated amino acids are attached to prosthetic group of carrier proteins by thioester bond. Weak thioacylation activity was observed for many conventional aaRS. Therefore, different thiols were investigated as substrate analogs for aa:CP ligases. Here we show that coenzyme A, dithiothreitol and cysteine are efficiently aminoacylated by aa:CP ligases. The crystal structure of aa:CP ligase from Bradyrhizobium japonicum in complex with coenzyme A was solved, and revealed that CoA, besides acting as the substrate analog of the carrier protein, also competes with ATP for binding to the active site. aa:CP ligases do not aminoacylate tRNA, although they remarkably resemble catalytic core of atypical seryl-tRNA synthetases (aSerRS) from methanogenic archaea. Since aa:CP ligases lack tRNA-binding domain, fusion proteins of aa:CP ligases and aSerRS tRNA-binding domain were prepared in attempt to restore tRNA aminoacylation activity. Although fusion proteins were able to bind tRNA through appended domain, tRNA could not substitute carrier proteins in aminoacylation reaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( POIROT)
119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi Hrčak

Citiraj ovu publikaciju:

Močibob, Marko; Ivić, Nives; Šubašić, Deni; Luić, Marija; Weygand-Đurašević, Ivana
Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases // Croatica chemica acta, 84 (2011), 2; 149-157 doi:10.5562/cca1818 (međunarodna recenzija, članak, znanstveni)
Močibob, M., Ivić, N., Šubašić, D., Luić, M. & Weygand-Đurašević, I. (2011) Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases. Croatica chemica acta, 84 (2), 149-157 doi:10.5562/cca1818.
@article{article, year = {2011}, pages = {149-157}, DOI = {10.5562/cca1818}, keywords = {aminoacyl-tRNA synthetase, amino acid:[carrier protein] ligase, carrier protein, coenzyme A, seryl-tRNA synthetase, thioester, tRNA}, journal = {Croatica chemica acta}, doi = {10.5562/cca1818}, volume = {84}, number = {2}, issn = {0011-1643}, title = {Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases}, keyword = {aminoacyl-tRNA synthetase, amino acid:[carrier protein] ligase, carrier protein, coenzyme A, seryl-tRNA synthetase, thioester, tRNA} }
@article{article, year = {2011}, pages = {149-157}, DOI = {10.5562/cca1818}, keywords = {aminoacyl-tRNA synthetase, amino acid:[carrier protein] ligase, carrier protein, coenzyme A, seryl-tRNA synthetase, thioester, tRNA}, journal = {Croatica chemica acta}, doi = {10.5562/cca1818}, volume = {84}, number = {2}, issn = {0011-1643}, title = {Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases}, keyword = {aminoacyl-tRNA synthetase, amino acid:[carrier protein] ligase, carrier protein, coenzyme A, seryl-tRNA synthetase, thioester, tRNA} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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