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Pregled bibliografske jedinice broj: 529681

Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus)


Klobučar, Goran I.V.; Malev, Olga; Šrut, Maja; Štambuk, Anamaria; Lorenzon, Simonetta; Cvetković, Želimira; Ferrero, Enrico A.; Maguire, Ivana
Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus) // Chemosphere, 87 (2012), 1; 62-67 doi:10.1016/j.chemosphere.2011.11.060 (međunarodna recenzija, članak, znanstveni)


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Naslov
Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus)

Autori
Klobučar, Goran I.V. ; Malev, Olga ; Šrut, Maja ; Štambuk, Anamaria ; Lorenzon, Simonetta ; Cvetković, Želimira ; Ferrero, Enrico A. ; Maguire, Ivana

Izvornik
Chemosphere (0045-6535) 87 (2012), 1; 62-67

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA damage; Comet assay; micronucleus test; narrow-clawed crayfish; haemocytes; cage exposure

Sažetak
Genotoxicity of freshwater pollution was assessed by measuring DNA damage in haemocytes of caged freshwater crayfish Astacus leptodactylus by the means of Comet assay and micronucleus test, integrated with the measurements of physiological (total protein concentration) and immunological (total haemocyte count) haemolymph parameters as biomarkers of undergone stress. Crayfish were collected at the reference site (River Mreţnica) and exposed in cages for one week at three polluted sites along the Sava River (Zagreb, Sisak, Krapje). The long term pollution status of these locations was confirmed by chemical analyses of sediments. Statistically significant increase in DNA damage measured by the Comet assay was observed at all three polluted sites comparing to the crayfish from reference site. In addition, native crayfish from the mildly polluted site (Krapje) cage exposed on another polluted site (Zagreb) showed lower DNA damage than crayfish from the reference site exposed at the same location indicating adaptation and acclimatisation of crayfish to lower levels of pollution. Micronuclei induction showed similar gradient of DNA damage as Comet assay, but did not reach the statistical significance. Observed increase in total haemocyte count and total protein content in crayfish from polluted environments in the Sava River also confirmed stress caused by exposure to pollution. The results of this study have proved the applicability of caging exposure of freshwater crayfish A. leptodactylus in environmental genotoxicity monitoring using Comet assay and micronucleus test.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( POIROT)

Ustanove:
Nastavni zavod za javno zdravstvo "Dr. Andrija Štampar"

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com ac.els-cdn.com dx.doi.org

Citiraj ovu publikaciju:

Klobučar, Goran I.V.; Malev, Olga; Šrut, Maja; Štambuk, Anamaria; Lorenzon, Simonetta; Cvetković, Želimira; Ferrero, Enrico A.; Maguire, Ivana
Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus) // Chemosphere, 87 (2012), 1; 62-67 doi:10.1016/j.chemosphere.2011.11.060 (međunarodna recenzija, članak, znanstveni)
Klobučar, G., Malev, O., Šrut, M., Štambuk, A., Lorenzon, S., Cvetković, Ž., Ferrero, E. & Maguire, I. (2012) Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus). Chemosphere, 87 (1), 62-67 doi:10.1016/j.chemosphere.2011.11.060.
@article{article, year = {2012}, pages = {62-67}, DOI = {10.1016/j.chemosphere.2011.11.060}, keywords = {DNA damage, Comet assay, micronucleus test, narrow-clawed crayfish, haemocytes, cage exposure}, journal = {Chemosphere}, doi = {10.1016/j.chemosphere.2011.11.060}, volume = {87}, number = {1}, issn = {0045-6535}, title = {Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus)}, keyword = {DNA damage, Comet assay, micronucleus test, narrow-clawed crayfish, haemocytes, cage exposure} }
@article{article, year = {2012}, pages = {62-67}, DOI = {10.1016/j.chemosphere.2011.11.060}, keywords = {DNA damage, Comet assay, micronucleus test, narrow-clawed crayfish, haemocytes, cage exposure}, journal = {Chemosphere}, doi = {10.1016/j.chemosphere.2011.11.060}, volume = {87}, number = {1}, issn = {0045-6535}, title = {Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus)}, keyword = {DNA damage, Comet assay, micronucleus test, narrow-clawed crayfish, haemocytes, cage exposure} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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