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Pregled bibliografske jedinice broj: 526038

Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein


Howard, Jamieson A. L.; Delmas, Stephane; Ivančić-Baće, Ivana; Bolt, Edward L.
Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein // Biochemical journal (London. 1984), 439 (2011), 1; 85-95 doi:10.1042/BJ20110901 (međunarodna recenzija, članak, znanstveni)


Naslov
Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein

Autori
Howard, Jamieson A. L. ; Delmas, Stephane ; Ivančić-Baće, Ivana ; Bolt, Edward L.

Izvornik
Biochemical journal (London. 1984) (0264-6021) 439 (2011), 1; 85-95

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Annealing; CRISPR-associated 3 (Cas3); CRISPR; helicase; R-loop

Sažetak
CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a nucleic acid processing system in bacteria and archaea that interacts with mobile genetic elements. CRISPR DNA and RNA sequences are processed by Cas proteins: in Escherichia coli K-12, one CRISPR locus links to eight cas genes (cas1, 2, 3 and casABCDE), whose protein products promote protection against phage. In the present paper, we report that purified E. coli Cas3 catalyses ATP-independent annealing of RNA with DNA forming R-loops, hybrids of RNA base-paired into duplex DNA. ATP abolishes Cas3 R-loop formation and instead powers Cas3 helicase unwinding of the invading RNA strand of a model R-loop substrate. R-loop formation by Cas3 requires magnesium as a co-factor and is inactivated by mutagenesis of a conserved amino acid motif. Cells expressing the mutant Cas3 protein are more sensitive to plaque formation by the phage λvir. A complex of CasABCDE ('Cascade') also promotes R-loop formation and we discuss possible overlapping roles of Cas3 and Cascade in E. coli, and the apparently antagonistic roles of Cas3 catalysing RNA-DNA annealing and ATP-dependent helicase unwinding.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
119-1191196-1201 - Organizacija, funkcija i mehanizmi evolucije biljnog genoma (Višnja Besendorfer, )

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Autor s matičnim brojem:
Ivana Ivančić Baće, (217264)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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