Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 524396

Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates


Rezić, Iva; Rolich, Tomislav
Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates // Tekstil : časopis za tekstilnu tehnologiju i konfekciju, 60 (2011), 2-3; 74-87 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 524396 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates

Autori
Rezić, Iva ; Rolich, Tomislav

Izvornik
Tekstil : časopis za tekstilnu tehnologiju i konfekciju (0492-5882) 60 (2011), 2-3; 74-87

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amino acids; image processing; MATLAB; metal ions; quantitative evaluation; scanning; TLC

Sažetak
This paper presents the first study of quantitative evaluation of thin layer chromatographic (TLC) plates by using a conventional scanner device and applying MATLAB program with Image Processing Toolbox. The program was used to quantify the colored image of developed thin layer chromatography plate which was then converted into gray scale with 256 levels of gray. Afterwards the desired threshold was chosen in order to transform the image into black and white, to determine the desired area, the borders and the coordinates of all spots on the plate. Finally, the density of the gray area was calculated for each object. Experiments showed that the obtained results of density and area of gray spot surfaces were proportional to the concentration values of analytes, covering a high concentration range with a linear correlation between program output and concentration of model ions. As model samples several different metal ions (namely cobalt, chromium and copper) were quantified on cellulose layer with the practical limits of error between 1 and 2 %. In addition, inter and intra plate precision, repeatability, relative standard deviation (RSD) and linearity were determined for all metal ions for regular and irregular shapes of spots.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Računarstvo



POVEZANOST RADA


Projekt / tema
117-1171419-1382 - Etika i ekologija u oplemenjivanju i njezi tekstila (Ivo Soljačić, )
117-0000000-3254 - Evolucijski algoritmi za optimiranje elektromagnetskog opterećenja okoliša (Darko Grundler, )

Ustanove
Tekstilno-tehnološki fakultet, Zagreb

Profili:

Avatar Url Tomislav Rolich (autor)

Avatar Url Iva Rezić (autor)

Citiraj ovu publikaciju

Rezić, Iva; Rolich, Tomislav
Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates // Tekstil : časopis za tekstilnu tehnologiju i konfekciju, 60 (2011), 2-3; 74-87 (međunarodna recenzija, članak, znanstveni)
Rezić, I. & Rolich, T. (2011) Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates. Tekstil : časopis za tekstilnu tehnologiju i konfekciju, 60 (2-3), 74-87.
@article{article, year = {2011}, pages = {74-87}, keywords = {amino acids, image processing, MATLAB, metal ions, quantitative evaluation, scanning, TLC}, journal = {Tekstil : \v{c}asopis za tekstilnu tehnologiju i konfekciju}, volume = {60}, number = {2-3}, issn = {0492-5882}, title = {Quantitative evaluation of chromatograms from scanned thin-layer chromatography plates}, keyword = {amino acids, image processing, MATLAB, metal ions, quantitative evaluation, scanning, TLC} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus