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Pregled bibliografske jedinice broj: 523845

Differential hepatoprotective mechanisms of rutin and quercetin in carbon tetrachloride-intoxicated BALB/cN mice


Domitrović, Robert; Jakovac, Hrvoje; Vasiljev Marchesi, Vanja; Vladimir-Knežević, Sanda; Cvijanović, Olga; Tadić, Žarko; Romić, Željko; Rahelić, Dario
Differential hepatoprotective mechanisms of rutin and quercetin in carbon tetrachloride-intoxicated BALB/cN mice // Acta pharmacologica Sinica, 33 (2012), 10; 1260-1270 doi:10.1038/aps.2012.62 (međunarodna recenzija, članak, znanstveni)


Naslov
Differential hepatoprotective mechanisms of rutin and quercetin in carbon tetrachloride-intoxicated BALB/cN mice

Autori
Domitrović, Robert ; Jakovac, Hrvoje ; Vasiljev Marchesi, Vanja ; Vladimir-Knežević, Sanda ; Cvijanović, Olga ; Tadić, Žarko ; Romić, Željko ; Rahelić, Dario

Izvornik
Acta pharmacologica Sinica (1671-4083) 33 (2012), 10; 1260-1270

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hepatotoxicity; rutin; quercetin; nuclear factor-kappaB; tumor necrosis factor-alpha; cyclooxygenase-2; nitric oxide synthase; NF-E2-related factor 2; heme oxygenase-1; transforming growth factor-beta1

Sažetak
Aim: To investigate the hepatoprotective mechanisms of flavonoid rutin against carbon tetrachloride (CCl4)-induced liver damage in mice and compare it with its aglycone quercetin. Methods: Rutin at doses of 10, 50, and 150 mg/kg and quercetin at 50 mg/kg were administered intraperitoneally into BALB/cN mice once daily for 5 consecutive days before intraperitoneal injection of CCl4. The animals were sacrificed 24 h later. Results: Pretreatment with rutin, and to the less extent, quercetin, significantly reduced the activity of plasma transaminases and improved histological signs of acute liver damage in CCl4- intoxicated mice. Quercetin prevented the decrease in Cu/Zn superoxide dismutase (SOD) activity in CCl4-intoxicated mice more potently than rutin, however, it was less effective in the suppression of nitrotyrosine (3-NT) formation. Quercetin, and to the less extent, rutin, significantly attenuated inflammation in the liver by down- regulating CCl4-induced activation of nuclear factor-kappaB (NF-κB), tumor necrosis factor-alpha (TNF-α), and cyclooxygenase (COX-2). The expression of inducible nitric oxide synthase (iNOS) was more potently suppressed by rutin than quercetin. Treatment with both flavonoids significantly increased NF-E2-related factor 2 (Nrf2) and heme oxygenase (HO-1) expression in injured livers, although quercetin was less effective than rutin at the equivalent dose. In contrast, transforming growth factor-beta1 (TGF- β1) expression was more suppressed by quercetin. Conclusions: Rutin provided stronger protection against nitrosative stress and hepatocellular damage but had weaker antioxidant and anti- inflammatory activity and antifibrotic potential than quercetin, which could be attributed to the presence of rutinoside moiety in position 3 of the C ring.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1238 - Bioaktivne prirodne tvari: izolacija, karakterizacija i biološki učinci (Sanda Vladimir-Knežević, )
062-0000000-3554 - Aktivni sastojci ljekovitog bilja u terapiji fibroze jetre (Robert Domitrović, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Rijeka,
Klinička bolnica "Dubrava",
Sveučilište u Rijeci

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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